protein

Recombinant human TNF-alpha protein

TNF0501

20 ug

55 USD

Recombinant human TNF-alpha protein

77-233aa

TNF0501

NP_000585.2

Tumor necrosis factor alpha (TNF-alpha), also called cachectin, consists of 157 amino acids. TNF-alpha is a 17. 5 kD factor produced by neutrophils, CD4+ T cells, macrophage, NK cells, LAK cells, astrocytes endothelial cells, and smooth muscle cells. TNF-alpha is cytolytic and plays an important role in immune regulation including hemorrhagic tumor necrosis/cytotoxicity and inflammation, and in regulation of antiviral and immune proliferative and activation responses. The active form of this protein is a trimer. Recombinant human TNF-alpha was expressed in E. coli and purified by using conventional chromatography techniques. Additional amino acid, methionine, was attached at N-terminus of the protein.

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

1mg/ml (determined by Bradford assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Chicoine M.R., et al. (1997), J. Neurooncol. 35: 249-57;; Ruddle N.H., et al. (1992), Curr. Opin. Immunol. 4: 327-32;; Wingfield P., et al. (1987), FEBS Lett. 211:179-184;;

> 95% by SDS-PAGE

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2ng/ml.

SDS-PAGE, Bioactivity

Human

Hormones & Cytokines

17.5 kDa (158aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Human TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL

Non-Tagged

E.coli

Recombinant Protein

P01375

TNF-a, Tumor necrosis factor alpha, TNFA, TNFSF2, DIF (differentiation inducing factor ), CACHECTIN, DIF, TNF-alpha, Tumor necrosis factor alpha, Tumor necrosis factor alpha, TNF, tumor necrosis factor (TNF superfamily, member 2), TNF superfamily member 2,TNF0501-10ug,TNF0501-20ug,TNF0501-50ug,TNF0501-100ug,TNF0501-250ug,TNF0501-500ug,TNF0501-1mg,TNF0501-10,TNF0501-20,TNF0501-50,TNF0501-100,TNF0501-250,TNF0501-500,TNF0501-1

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