Recombinant streptomyces avidinii Streptavidin protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant streptomyces avidinii Streptavidin protein

catalog :

STV0801

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant streptomyces avidinii Streptavidin protein

Domain :

25-183aa

Catalog number (SKU) :

STV0801

NCBI :

CAA27265.1

Description :

Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant His tagged streptavidin was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Streptavidin, Streptomyces avidinii streptavidin, SA V1, SA V2, Streptavidin V1, Streptavidin V2.

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 7.5)

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Howarth M., et al. (2006) Nat Methods. 3(4):267-73.;; Holmberg A., et al (2005). Electrophoresis. 26(3):501-10.;;

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Streptomyces, Streptomyces avidinii

Gene Category :

Fusion Tag & Detection

Molecular Weight :

17.5 kDa (167aa) confirmed by MALDI-TOF

Amino Acid Sequence :

DP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P22629

Internal Search Terms :

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more info or order :

NKMAX product webpage