Recombinant E.coli SlyD protein | NKMAX SLD0801 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant E.coli SlyD protein

catalog :

SLD0801

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant E.coli SlyD protein

Domain :

1-196aa

Catalog number (SKU) :

SLD0801

NCBI :

NP_755987

Description :

SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase. Recombinant E. Coli slyD was expressed in E. coli and purified by conventional chromatography.

Alternative Names :

FKBP-type peptidyl-prolyl cis-trans isomerase, FKBP-type peptidyl-prolyl cis-trans isomerase, SlyD, FKBP-type peptidyl-prolyl cis-trans isomerase FKBP type peptidyl prolyl cis trans isomerase slyD, Histidine rich protein, PPIase, Rotamase, WHP.

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 7.5)

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Zhang JW., et al. (2007) J Bacteriol. 189(21):7942-4.;; Scholz C., et al. (2006) Biochemistry. 45(1):20-33.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 700nmol/min/mg, and is defined as the amount of enzyme cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

E.coli

Gene Category :

Heat Shock Proteins

Molecular Weight :

21 kDa (196aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Amino Acid Sequence :

MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P0A9K9

Internal Search Terms :

SlyD, FKBP-type peptidyl-prolyl cis-trans isomerase, FKBP-type peptidyl-prolyl cis-trans isomerase, SlyD, FKBP-type peptidyl-prolyl cis-trans isomerase FKBP type peptidyl prolyl cis trans isomerase slyD, Histidine rich protein, PPIase, Rotamase, WHP.,SLD0801-10ug,SLD0801-20ug,SLD0801-50ug,SLD0801-100ug,SLD0801-250ug,SLD0801-500ug,SLD0801-1mg,SLD0801-10,SLD0801-20,SLD0801-50,SLD0801-100,SLD0801-250,SLD0801-500,SLD0801-1

more info or order :

NKMAX product webpage