protein

Recombinant human SHP-1 protein

SHP2001

20 ug

105 USD

Recombinant human SHP-1 protein

243-541aa

SHP2001

NP_002822

The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541) was cloned into an E. coli expression vector. The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). The recombinant SHP-1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid (Met) is attached at N-terminus.

Protein tyrosine phosphatase non-receptor type 6 isoform 1, PTPN6, HCP, PTP1C, Tyrosine-protein phosphatase non-receptor type 6, EC 3.1.3.48, Protein-tyrosine phosphatase 1C, PTP-1C, Hematopoietic cell protein-tyrosine phosphatase, SH-PTP1, Protein-tyrosine phosphatase SHP-1, PTPN6, HCP, HCPH, SHP1, HPTP1C, SHP-1L EC 3.1.3.48, Protein tyrosine phosphatase non-receptor type 6 isoform 1, 70 kda SHP1L protein, Hematopoietic cell phosphatase, Hematopoietic cell protein tyrosine phosphatase, HPTP 1C, Protein tyrosine phosphatase 1C, Protein tyrosine phosphatase SHP 1, Protein tyrosine phosphatase SHP1, PTP 1C, PTPN 6, SH PTP 1, SH PTP1, SHP 1, SHP 1L, SHP1L, SHPTP 1, SHPTP1, Tyrosine protein phosphatase non receptor type 6

1mg/ml (determined by Bradford assay)

Liquid in. 25mM Tris-HCl buffer (pH 7.5) containing 2mM beta-mercaptoethanol, 1mM EDTA 1mM DTT, 20%glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Shen SH., et al. (1991) Nature 352, 736-739.;; Wu C., et al. (2003) Gene. 306,1-12.;;

> 95% by SDS-PAGE

Specific activity is > 8,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C

SDS-PAGE, Enzyme Activity

Human

Signal Transduction

34.3 kDa (300aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 8,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C

MGFWEEFESL QKQEVKNLHQ RLEGQRPENK GKNRYKNILP FDHSRVILQG RDSNIPGSDY INANYIKNQL LGPDENAKTY IASQGCLEAT VNDFWQMAWQ ENSRVIVMTT REVEKGRNKC VPYWPEVGMQ RAYGPYSVTN CGEHDTTEYK LRTLQVSPLD NGDLIREIWH YQYLSWPDHG VPSEPGGVLS FLDQINQRQE SLPHAGPIIV HCSAGIGRTG TIIVIDMLME NISTKGLDCD IDIQKTIQMV RAQRSGMVQT EAQYKFIYVA IAQFIETTKK KLEVLQSQKG QESEYGNITY

Non-Tagged

E.coli

Recombinant Protein

P29350

SHP1,SHP2001-10ug,SHP2001-20ug,SHP2001-50ug,SHP2001-100ug,SHP2001-250ug,SHP2001-500ug,SHP2001-1mg,SHP2001-10,SHP2001-20,SHP2001-50,SHP2001-100,SHP2001-250,SHP2001-500,SHP2001-1