protein

Recombinant human SETD7 protein

SET3001

20 ug

55 USD

Recombinant human SETD7 protein

1-366aa

SET3001

NP_085151

SET 7/9 is a histone methyltransferase (HMTase) that transfers methyl groups to Lys4 of histone H3, in complex with S-adenosyl-L-methionine (AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression. Acetylation, phosphorylation and methylation of the amino-terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. SET 7/9, unlike most other SET proteins, is exclusively a mono-methylase, Recombinant human SET7/9, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.

SETD7, SET7, SET9, SET7/9, SET7/9 Histone methyltransferase, SET domain-containing protein 8, SET domain-containing protein 7 FLJ21193, SET domain-containing protein 7, Lysine N-methyltransferase 7, Lysine methyltransferase, KMT7, KIAA1717, Histone-lysine N-methyltransferase SETD7, Histone-lysine N-methyltransferase, Histone lysine N methyltransferase H3 lysine 4 specific SET7, Histone lysine methyltransferase, Histone H4-K4 methyltransferase, Histone H3-K4 methyltransferase SETD7, Histone H3 lysine 4 specific methyltransferase, Histone H3 K4 methyltransferase, H4 lysine-4 specific, H3-K4-HMTase SETD7, H3 K4 HMTase, EC 2.1.1.43

1mg/ml (determined by Bradford assay)

Liquid in. 50mM Tris-HCl buffer (pH 7.5) containing 0.2M NaCl, 5mM DTT, 20%glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Xiao B., et al. (2003) Nature. 421(6923):652-6.;; Kwon T., et al. (2003) EMBO J. 22, 292-303.;;

> 95% by SDS-PAGE

SDS-PAGE

Human

Histone Related

40.7 kDa (366aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

MDSDDEMVEE AVEGHLDDDG LPHGFCTVTY SSTDRFEGNF VHGEKNGRGK FFFFDGSTLE GYYVDDALQG QGVYTYEDGG VLQGTYVDGE LNGPAQEYDT DGRLIFKGQY KDNIRHGVCW IYYPDGGSLV GEVNEDGEMT GEKIAYVYPD ERTALYGKFI DGEMIEGKLA TLMSTEEGRP HFELMPGNSV YHFDKSTSSC ISTNALLPDP YESERVYVAE SLISSAGEGL FSKVAVGPNT VMSFYNGVRI THQEVDSRDW ALNGNTLSLD EETVIDVPEP YNHVSKYCAS LGHKANHSFT PNCIYDMFVH PRFGPIKCIR TLRAVEADEE LTVAYGYDHS PPGKSGPEAP EWYQVELKAF QATQQK

Non-Tagged

E.coli

Recombinant Protein

Q8WTS6

SETD7, SET9, SET7/9 Histone methyltransferase, SET7/9, SET7, SET domain-containing protein 8, SET domain-containing protein 7 FLJ21193, SET domain-containing protein 7, SET domain containing protein 7, SET D7, SET 9, SET 7/9, SET 7, Lysine N-methyltransferase 7, Lysine methyltransferase, KMT7, KIAA1717, Histone-lysine N-methyltransferase SETD7, Histone-lysine N-methyltransferase, Histone lysine N methyltransferase H3 lysine 4 specific SET7, Histone lysine methyltransferase, Histone H4-K4 methyltransferase, Histone H3-K4 methyltransferase SETD7, Histone H3 lysine 4 specific methyltransferase, Histone H3 K4 methyltransferase, H4 lysine-4 specific, H3-K4-HMTase SETD7, H3 K4 HMTase, EC 2.1.1.43, SET3001-10ug, SET3001-20ug, SET3001-50ug, SET3001-100ug, SET3001-250ug, SET3001-500ug, SET3001-1mg, SET3001-010, SET3001-020, SET3001-050, SET3001-100, SET3001-250, SET3001-500, SET3001-01M

Choi KC, et al. Gallic acid suppresses lipopolysaccharide-induced nuclear factor-kappaB signaling by preventing RelA acetylation in A549 lung cancer cells. (Mol Cancer Res. 2009){https://pubmed.ncbi.nlm.nih.gov/19996305/}