Recombinant human DJ-1/PARK7 protein | NKMAX PPR0801 product information

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company name :

NKMAX

product type :

protein

product name :

Recombinant human DJ-1/PARK7 protein

catalog :

PPR0801

quantity :

20 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human DJ-1/PARK7 protein

Domain :

1-147aa

Catalog number (SKU) :

PPR0801

NCBI :

NP_150281

Description :

PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization. Recombinant His tagged PPP1R14A was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Regulatory subunit 14A, Regulatory (inhibitor) subunit 14A CPI 17, Regulatory (inhibitor) subunit 14A, Protein phosphatase 1 regulatory subunit 14A, Protein phosphatase 1 regulatory (inhibitor) subunit 14A, Protein phosphatase 1, PPP1R14A, PPP1INL, PKC potentiated inhibitory protein of PP1, CPI-17, CPI 17 alpha

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 2mM EDTA, 1mM DTT, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Hamaguchi., et al. (2000). Biochem. Biophys. Res Commun. 274(3):825-30.;; Eto M., et al. (1997). FEBS Lett. 410(2-3):356-60.;; Lartey I., et al. (2007). Biol Reprod. 76(6):971-82.;;

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Signal Transduction

Molecular Weight :

18 kDa (167aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL DVEKWIDGRL EELYRGMEAD MPDEINIDEL LELESEEERS RKIQGLLKSC GKPVEDFIQE LLAKLQGLHR QPGLRQPSPS HDGSLSPLQD RARTAHP

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q96A00

Internal Search Terms :

Regulatory subunit 14A, Regulatory (inhibitor) subunit 14A CPI 17, Regulatory (inhibitor) subunit 14A, Protein phosphatase 1 regulatory subunit 14A, Protein phosphatase 1 regulatory (inhibitor) subunit 14A, Protein phosphatase 1, PPP1R14A, PPP1INL, PKC potentiated inhibitory protein of PP1, CPI-17, CPI17, CPI 17alpha, CPI 17 alpha, CPI-17 alpha, CPI17 alpha, PPR0801-10ug, PPR0801-20ug, PPR0801-50ug, PPR0801-100ug, PPR0801-250ug, PPR0801-500ug, PPR0801-1mg, PPR0801-010, PPR0801-020, PPR0801-050, PPR0801-100, PPR0801-250, PPR0801-500, PPR0801-01M

more info or order :

NKMAX product webpage