protein

Recombinant human Cyclophilin H/PPIH protein

PPH0901

20 ug

105 USD

Recombinant human Cyclophilin H/PPIH protein

1-177aa

PPH0901

NP_006338

Cyclophilin H (also known as peptidylpropyl isomerase H, PPIH) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. The cyclophilin H is a specific component of the human u4/u6 small nuclear ribonucleoprotein particle involved in the nuclear splicing of pre-mRNA. It stably associates with the u4/u6-60kD and -90kD proteins, the human orthologues of the Saccharomyces cerevisiae Prp4 and Prp3 splicing factors. Recombinant human cyclophilin H was expressed in E. coli and purified by conventional chromatography techniques.

Peptidylprolyl isomerase H, Peptidylprolyl isomerase H, PPIH, CYPH, CYP20, SnuCyp-20, SnuCyp-20, PPIase H, Rotamase H, Cyclophilin H, Peptidylprolyl isomerase H CypH, EC 5.2.1.8, MGC5016, Small nuclear ribonucleoprotein particle-specific cyclophilin H, uSACYP, usnRNP associated cyclophilin SnuCyp20,

1mg/ml (determined by Bradford assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Reidt u., et al. (2000) J Biol Chem. 275(11):7439-42.;; Horowitz DS., et al. (2002) EMBO. 21(3):470-80.;;

> 95% by SDS-PAGE

Specific activity is > 220nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin.

SDS-PAGE, Enzyme Activity

Human

Cell Cycle

19.2 kDa (177aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 220 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin.

MAVANSSPVN PVVFFDVSIG GQEVGRMKIE LFADVVPKTA ENFRQFCTGE FRKDGVPIGY KGSTFHRVIK DFMIQGGDFV NGDGTGVASI YRGPFADENF KLRHSAPGLL SMANSGPSTN GCQFFITCSK CDWLDGKHVV FGKIIDGLLV MRKIENVPTG PNNKPKLPVV ISQCGEM

Non-Tagged

E.coli

Recombinant Protein

O43447

Cyclophilin H, PPIH, Peptidylprolyl isomerase H, Peptidylprolyl isomerase H, PPIH, CYPH, CYP20, SnuCyp-20, SnuCyp-20, PPIase H, Rotamase H, Cyclophilin H, Peptidylprolyl isomerase H CypH, EC 5.2.1.8, MGC5016, Small nuclear ribonucleoprotein particle-specific cyclophilin H, uSACYP, usnRNP associated cyclophilin SnuCyp20,PPH0901-10ug,PPH0901-20ug,PPH0901-50ug,PPH0901-100ug,PPH0901-250ug,PPH0901-500ug,PPH0901-1mg,PPH0901-10,PPH0901-20,PPH0901-50,PPH0901-100,PPH0901-250,PPH0901-500,PPH0901-1