protein

Recombinant human Cyclophilin F/PPIF protein

PPF0901

20 ug

55 USD

Recombinant human Cyclophilin F/PPIF protein

30-207aa

PPF0901

NP_005720

Cyclophilin F (also known as Peptidylprolyl isomerase F, PPIF) is a member of peptidyl-prolyl cis-trans isomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. Recombinant PPIF protein was expressed in E. coli and purified by using conventional chromatography techniques.

peptidylprolyl isomerase F, Peptidyl-prolyl cis-trans isomerase F mitochondrial, PPIase F, Cyclophilin D, CyP-D, CypD, Cyclophilin F, Mitochondrial cyclophilin, CyP-M, Rotamase F

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bergsma DJ., et al. (1991) J Biol Chem. 266(34):23204-14.;; Bowles KR., et al. (2000) Genomics 67(2):109-27.

> 95% by SDS-PAGE

Specific activity is > 900nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

SDS-PAGE, Enzyme Activity

Human

Enzymes & Proteases

21.1 kDa (199aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 900 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

MGSSHHHHHH SSGLVPRGSH MCSKGSGDPS SSSSSGNPLV YLDVDANGKP LGRVVLELKA DVVPKTAENF RALCTGEKGF GYKGSTFHRV IPSFMCQAGD FTNHNGTGGK SIYGSRFPDE NFTLKHVGPG VLSMANAGPN TNGSQFFICT IKTDWLDGKH VVFGHVKEGM DVVKKIESFG SKSGRTSKKI VITDCGQLS

His-Tag

E.coli

Recombinant Protein

P30405

Cyclophilin D, Cyclophilin F, Cyp D, CYP3, CypD, Cyp-D, hCyP3, mitochondral, Peptidyl prolyl cis trans isomerase, Peptidylprolyl isomerase F, Peptidylprolyl isomerase F (cyclophilin F), Peptidylprolyl isomerase F Cyclophilin 3, PPIase, PPIF, Rotamase, PPF0901-10ug, PPF0901-20ug, PPF0901-50ug, PPF0901-100ug, PPF0901-250ug, PPF0901-500ug, PPF0901-1mg, PPF0901-010, PPF0901-020, PPF0901-050, PPF0901-100, PPF0901-250, PPF0901-500, PPF0901-01M

Gallay PA, et al. Characterization of the Anti-HCV Activities of the New Cyclophilin Inhibitor STG-175. (PLoS One. 2016){https://pubmed.ncbi.nlm.nih.gov/27104614/};;Kon N, et al. A small-molecule DS44170716 inhibits Ca 2+-induced mitochondrial permeability transition. (Sci Rep. 2017){https://pubmed.ncbi.nlm.nih.gov/28634393/}