protein

Recombinant human PP2C alpha/PPM1A protein

PPC3001

20 ug

55 USD

Recombinant human PP2C alpha/PPM1A protein

1-382aa

PPC3001

NP_066283

Protein phosphatase 2C (PP2C alpha) is a Mn2+- or Mg2+- dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. The protein coding region of PP2C alpha (amino acids 1-382) was cloned into an E. coli expression vector. PP2C alpha was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques.

Pyruvate dehydrogenase phosphatase catalytic subunit 1, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1, Protein phosphatase Mg2+/Mn2+dependent 1A, Protein phosphatase 2C, Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform, Protein phosphatase 1A (formerly 2C), PPM2C, PPM1A, PP2CA, PP2C alpha, PDPC 1, PDP 1, PDP, MGC9201, FLJ42306, EC 3.1.3.43

1mg/ml (determined by Bradford assay)

Liquid in. 10mM Tris-HCl buffer (pH 7.5) containing 50mM NaCl, 2mM DTT, 1mM MnCl2, 20%glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Das, A. K. et al. (1996) EMBO J. 15, 6798-6809

> 95% by SDS-PAGE

Specific activity is > 1,400unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

SDS-PAGE, Enzyme Activity

Human

Signal Transduction

46.6 kDa (418aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 1,400 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

MGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW

His-Tag

E.coli

Recombinant Protein

P35813

Pyruvate dehydrogenase phosphatase catalytic subunit 1, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1, Protein phosphatase Mg2+/Mn2+dependent 1A, Protein phosphatase 2C, Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform, Protein phosphatase 1A (formerly 2C), PPM2C, PPM1A, PP2C-alpha, PP2Calpha, PP2CA, PP2C A, PP2C alpha, PDPC 1, PDP1, PDP 1, PDP, MGC9201, FLJ42306, EC 3.1.3.43, PPC3001-10ug, PPC3001-20ug, PPC3001-50ug, PPC3001-100ug, PPC3001-250ug, PPC3001-500ug, PPC3001-1mg, PPC3001-010, PPC3001-020, PPC3001-050, PPC3001-100, PPC3001-250, PPC3001-500, PPC3001-01M