protein

Recombinant human Pin1 protein

PIN3001

20 ug

80 USD

Recombinant human Pin1 protein

1-163aa

PIN3001

NP_006212.1

Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA, PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.

Protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1, PIN1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC 5.2.1.8, Rotamase Pin1, PPIase Pin1, DOD, uBL5, PIN1, PPIase, EC 5.2.1.8, Rotamase Pin1, PPIase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 7.5) 0.1M NaCl, 5mM DTT, 20%glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Wulf GM., et al. (2002) J Biol. Chem. 277(50):47976-47979;; Hamdane M., et al. (2002) J Mol Neurosci. 19(3): 275-287;; Zheng H, et al. (2002) Nature. 419(6909) 853-857;;

> 95% by SDS-PAGE

Specific activity is > 1,200nmol/min/mg, and is defined as the amount of enzyme that cleaves of suc-AAPF-pNA per minute at 37C.

SDS-PAGE, Enzyme Activity

Human

Cell Cycle

18.2 kDa (163aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 1,200 nmol/min/mg, and is defined as the amount of enzyme that cleaves of suc-AAPF-pNA per minute at 37C.

MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE

Non-Tagged

E.coli

Recombinant Protein

Q13526

Pin1,PIN3001-10ug,PIN3001-20ug,PIN3001-50ug,PIN3001-100ug,PIN3001-250ug,PIN3001-500ug,PIN3001-1mg,PIN3001-10,PIN3001-20,PIN3001-50,PIN3001-100,PIN3001-250,PIN3001-500,PIN3001-1

Melino S, et al. p63 threonine phosphorylation signals the interaction with the WW domain of the E3 ligase Itch. (Cell Cycle. 2014){https://pubmed.ncbi.nlm.nih.gov/25485500/}