protein

Recombinant human PCNA protein

PDN0801

20 ug

80 USD

Recombinant human PCNA protein

1-261aa

PDN0801

NP_002583.1

PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by uV irradiation, growth factors and eventually by neighbouring tumours. Recombinant human PCNA, was expressed in E. coli and purified by conventional chromatography techniques.

Proliferating cell nuclear antigen, MGC8367, Proliferating cell nuclear antigen, Proliferating cell nuclear antigen, PCNA,

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 2mM EDTA, 20% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Matsumoto K., et al. (1987) EMBO J. 6(3):637-42. ;; Moldovan GL., et al. (2007). Cell. 129(4):665-79.;;

> 95% by SDS-PAGE

SDS-PAGE

Human

Cell Cycle

28.7 kDa (261aa) confirmed by MALDI-TOF

MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S

Non-Tagged

E.coli

Recombinant Protein

P12004

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