protein

Recombinant human Serpin E1/PAI-1 protein

PAI0701

20 ug

80 USD

Recombinant human Serpin E1/PAI-1 protein

24-402aa

PAI0701

NP_000593

Plasminogen activator inhibitor-1 (PAI-1), a member of the serine protease inhibitor (serpin) super family. PAI-1 is the principal inhibitor of tissue plasminogen activator and urokinase, the activators of plasminogen and hence fibrinolysis. PAI-1 is mainly produced by the endothelium but is also secreted by other tissue types, such as adipose tissue. Recombinant human PAI-1, fused to His tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Plasminogen activator inhibitor-1, PLANH1, SERPINE1, PAI1, PLANH1, Plasminogen activator inhibitor-1, Plasminogen activator inhibitor-1

1mg/ml (determined by Bradford assay)

Liquid in. 50mM NaAc (pH 5.5) containing 10% glycerol, 0.1M NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Gorlatova NV., et al.(2007) J Biol Chem. 282(12):9288-96.;; Renckens R., et al. (2006) J Immunol. 177(11):8171-6.;;

> 95% by SDS-PAGE

The IC50 for this effect is less than 3 nM, Measured by its ability to inhibit uPA cleavage of the substrate Z-GGR-AMC.

SDS-PAGE, Enzyme Activity

Human

Enzymes & Proteases

45 kDa (400aa)

The IC50 for this effect is less than 3 nM, Measured by its ability to inhibit uPA cleavage of the substrate Z-GGR-AMC.

MGSSHHHHHH SSGLVPRGSH MVHHPPSYVA HLASDFGVRV FQQVAQASKD RNVVFSPYGV ASVLAMLQLT TGGETQQQIQ AAMGFKIDDK GMAPALRHLY KELMGPWNKD EISTTDAIFV QRDLKLVQGF MPHFFRLFRS TVKQVDFSEV ERARFIINDW VKTHTKGMIS NLLGKGAVDQ LTRLVLVNAL YFNGQWKTPF PDSSTHRRLF HKSDGSTVSV PMMAQTNKFN YTEFTTPDGH YYDILELPYH GDTLSMFIAA PYEKEVPLSA LTNILSAQLI SHWKGNMTRL PRLLVLPKFS LETEVDLRKP LENLGMTDMF RQFQADFTSL SDQEPLHVAQ ALQKVKIEVN ESGTVASSST AVIVSARMAP EEIIMDRPFL FVVRHNPTGT VLFMGQVMEP

His-Tag

E.coli

Recombinant Protein

P05121

Serpin E1, PAI1, Plasminogen activator inhibitor-1, PLANH1, SERPINE1, PAI1, PLANH1, Plasminogen activator inhibitor-1, Plasminogen activator inhibitor-1,PAI0701-10ug,PAI0701-20ug,PAI0701-50ug,PAI0701-100ug,PAI0701-250ug,PAI0701-500ug,PAI0701-1mg,PAI0701-10,PAI0701-20,PAI0701-50,PAI0701-100,PAI0701-250,PAI0701-500,PAI0701-1

Cho KS, et al. Valproic acid induces astrocyte-dependent neurite outgrowth from cultured rat primary cortical neuron via modulation of tPA/PAI-1 activity. (Glia. 2013){https://pubmed.ncbi.nlm.nih.gov/23378038/}