Recombinant human OPG/TNFRSF11B protein | NKMAX OPG0905 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human OPG/TNFRSF11B protein

catalog :

OPG0905

quantity :

10 ug

price :

81 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human OPG/TNFRSF11B protein

Domain :

22-401aa

Catalog number (SKU) :

OPG0905

NCBI :

NP_002537.3

Description :

Osteoprotegerin (OPG) is a member of the tumor necrosis factor (TNF) -related family, is referred to as TNFRSF11B and part of the OPG/receptor activator of NF-kB ligand (RANKL) /receptor activator of NF-kB (RANK) triad. This cytokine that lacks any apparent cell-association motifs and exists as a soluble secreted protein, network regulates the differentiation and activation of osteoclasts and hence the critical balance between bone formation (osteoblasts) and bone resorption (osteoclasts). Recombinant human osteoprotegerin protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography techniques.

Alternative Names :

Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin, OCIF, OPG, TR1

Concentration :

0.5 mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Lacey, D.L. et al. (1998) Cell 93:165-76;; Yasuda H. et al. (1998) PNAS 95:3597-602 ;; A. Van Campenhout, Golledge J. (2009) Atherosclerosis 204:321-29;;

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Measured by its ability to inhibit cytotoxicity using Jurkat human acute T cell leukemia cells in the presence of 2ng/ml of human TRAIL (CAT# ATGP3588). The ED50 range ≤ 8 ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Hormones & Cytokines

Molecular Weight :

44.7 kDa (389aa) ;;(On SDS-PAGE under denatured condition, apparent Molecular weight of glycosylated rhOPG protein will appear at approximately 55 kDa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human Human OPG/TNFRSF11B inhibits Human TRAIL (CAT# ATGP3588) cytotoxicity in the Jurkat human acute T cell leukemia cells. The ED50 range ≤ 8 ng/ml.

Amino Acid Sequence :

ETFPPKY LHYDEETSHQ LLCDKCPPGT YLKQHCTAKW KTVCAPCPDH YYTDSWHTSD ECLYCSPVCK ELQYVKQECN RTHNRVCECK EGRYLEIEFC LKHRSCPPGF GVVQAGTPER NTVCKRCPDG FFSNETSSKA PCRKHTNCSV FGLLLTQKGN ATHDNICSGN SESTQKCGID VTLCEEAFFR FAVPTKFTPN WLSVLVDNLP GTKVNAESVE RIKRQHSSQE QTFQLLKLWK HQNKDQDIVK KIIQDIDLCE NSVQRHIGHA NLTFEQLRSL MESLPGKKVG AEDIEKTIKA CKPSDQILKL LSLWRIKNGD QDTLKGLMHA LKHSKTYHFP KTVTQSLKKT IRFLHSFTMY KLYQKLFLEM IGNQVQSVKI SCL

Tag :

His-Tag

Expression System :

Baculovirus

Product Type Name :

Recombinant Protein

UniProt No. :

O00300

Internal Search Terms :

Osteoprotegerin, TNFRSF11B, Tumor necrosis factor receptor superfamily member 11b, Osteoclastogenesis inhibitory factor, OPG, OCIF, Tumor necrosis factor receptor superfamily, member 11b MGC29565, TNFRSF 11B, TNFRSF11B, TR 1, TR1, Tumor Necrosis Factor Receptor Superfamily Member 11b.,OPG0905-10ug,OPG0905-20ug,OPG0905-50ug,OPG0905-100ug,OPG0905-250ug,OPG0905-500ug,OPG0905-1mg,OPG0905-10,OPG0905-20,OPG0905-50,OPG0905-100,OPG0905-250,OPG0905-500,OPG0905-1

more info or order :

NKMAX product webpage