protein

Recombinant human Enolase 2/ENO2 protein

NSE0801

20 ug

80 USD

Recombinant human Enolase 2/ENO2 protein

1-434aa

NSE0801

NP_001966.1

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E. coli and purified by conventional chromatography techniques.

Enolase 2 (gamma, neuronal), ENO2, NSE, Neuron-Specific Enolase, 2 phospho D glycerate hydrolyase, Eno 2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma enolase, Neural enolase, Neuron specific enolase, Neuron specific gamma enolase, Neurone specific enolase.

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 0.1 M KCl, 5mM MgSO4

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

T Kirino., et al.(1983). J. Neuroscience. 3, 915-923;; Johnson DH., et al.(1984). Cancer Res. 44(11):5409-14;;

> 95% by SDS-PAGE

Specific activity is > 25,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.

SDS-PAGE, Enzyme Activity

Human

Neuroscience

47.2 kDa ( 434aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 25,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.

MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL

Non-Tagged

E.coli

Recombinant Protein

P09104

Enolase2, Neuron-specific Enolase, Enolase 2 (gamma, neuronal), ENO2, NSE, Neuron-Specific Enolase, 2 phospho D glycerate hydrolyase, Eno 2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma enolase, Neural enolase, Neuron specific enolase, Neuron specific gamma enolase, Neurone specific enolase.,NSE0801-10ug,NSE0801-20ug,NSE0801-50ug,NSE0801-100ug,NSE0801-250ug,NSE0801-500ug,NSE0801-1mg,NSE0801-10,NSE0801-20,NSE0801-50,NSE0801-100,NSE0801-250,NSE0801-500,NSE0801-1

Nakamura K, et al. Proteome analysis of cerebrospinal fluid in healthy beagles and canine encephalitis. (J Vet Med Sci. 2012){https://pubmed.ncbi.nlm.nih.gov/22251802/}