Recombinant human NEDD8 protein | NKMAX NED0905 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human NEDD8 protein

catalog :

NED0905

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human NEDD8 protein

Domain :

1-76aa

Catalog number (SKU) :

NED0905

NCBI :

NP_006147

Description :

NEDD8 belongs to the ubiquitin family and human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The NEDD8 system is essential for the regulation of protein degradation pathways involved in cell cycle progression, morphogenesis and tumorigenesis. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Recombinant human NEDD8 protein was expressed in E. coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer.

Alternative Names :

Neural precursor cell expressed developmentally down-regulated 8, ubiquitin-like protein Nedd8, Neddylin, Neural precursor cell expressed, developmentally down-regulated 8 MGC104393, MGC125896, MGC125897, NED8, 0NEDD 8, Neddylin, Neural precursor cell expressed developmentally down regulated 8, Neural precursor cell expressed developmentally down regulated gene 8, Rub1, ubiquitin like protein Nedd 8, ubiquitin like protein Nedd8.

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.5mM DTT, 50mM NaCl, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Wu K., et al. (2002) J Biol Chem. 277(1): 516-27;; Hori T., et al. (1999) Oncogene. 18(48): 6829-34;;

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Ubiquitination

Molecular Weight :

12.8 kDa (113aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Amino Acid Sequence :

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMLI KVKTLTGKEI EIDIEPTDKV ERIKERVEEK EGIPPQQQRL IYSGKQMNDE KTAADYKILG GSVLHLVLAL RGG

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q15843

Internal Search Terms :

NEDD8, Neural precursor cell expressed developmentally down-regulated 8, ubiquitin-like protein Nedd8, Neddylin, Neural precursor cell expressed, developmentally down-regulated 8 MGC104393, MGC125896, MGC125897, NED8, 0NEDD 8, Neddylin, Neural precursor cell expressed developmentally down regulated 8, Neural precursor cell expressed developmentally down regulated gene 8, Rub1, ubiquitin like protein Nedd 8, ubiquitin like protein Nedd8.,NED0905-10ug,NED0905-20ug,NED0905-50ug,NED0905-100ug,NED0905-250ug,NED0905-500ug,NED0905-1mg,NED0905-10,NED0905-20,NED0905-50,NED0905-100,NED0905-250,NED0905-500,NED0905-1

more info or order :

NKMAX product webpage