protein

Recombinant mouse GM-CSF protein

MSF0801

10 ug

110 USD

Recombinant mouse GM-CSF protein

18-141aa

MSF0801

NP_034099

GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Mouse GM-CSF protein is 125 amino acids and shares approximately 60% homology with the human GM-CSF protein. Recombinant mouse GM-CSF was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer

Granulocyte-macrophage colony stimulating factor 2, Csf2, Csfgm, MGI-IGM, mGM-CSF, Granulocyte-macrophage colony stimulating factor 2, Granulocyte-macrophage colony stimulating factor 2

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Fleetwood AJ., et al , (2005). Crit Rev Immunol. 25(5):405-28.;; Hamilton JA., et al. (2004). Growth Factors. 22(4):225-31.;;

> 95% by SDS-PAGE

Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 for this effect is less or equal to 0.06ng/ml.

SDS-PAGE, Bioactivity

Mouse

Hormones & Cytokines

14 kDa (125aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Mouse GM-CSF stimulates cell proliferation of the MC/9 mouse mast cells. The ED50 range is less or equal 0.06 ng/ml.

MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK

Non-Tagged

E.coli

Recombinant Protein

P01587

GM-CSF,MSF0801-10ug,MSF0801-20ug,MSF0801-50ug,MSF0801-100ug,MSF0801-250ug,MSF0801-500ug,MSF0801-1mg,MSF0801-10,MSF0801-20,MSF0801-50,MSF0801-100,MSF0801-250,MSF0801-500,MSF0801-1

Hotta M, et al. GM-CSF therapy inhibits chronic graft-versus-host disease via expansion of regulatory T cells. Eur J Immunol. 2019 Jan;49(1):179-191.{https://pubmed.ncbi.nlm.nih.gov/30457669/};;Tsubokura Y, et al. Early administration of lenalidomide after allogeneic hematopoietic stem cell transplantation suppresses graft-versus-host disease by inhibiting T-cell migration to the gastrointestinal tract. (Immun Inflamm Dis. 2022 Sep){https://pubmed.ncbi.nlm.nih.gov/36039651/}