protein

Recombinant human GM-CSF protein

MSF0501

10 ug

120 USD

Recombinant human GM-CSF protein

18-144aa

MSF0501

NP_000749

Human GM-CSF is a 14. 6 kDa globular protein consisting of 128 amino acid containing two dislufied bonds. GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Recombinant human GM-CSF was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Granulocyte-macrophage colony stimulating factor, CSF2, GMCSF, GM-CSF, GM-CSF, Colony-stimulating factor, CSF, Sargramostim, Molgramostin, Granulocyte-macrophage colony-stimulating factor, Granulocyte-macrophage colony stimulating factor Burst Promoting Activity, CMCSF, Colony stimulating factor, Colony Stimulating Factor 2, CSF 2, CSF Alpha, Eosinophil Colony Stimulating Factor,Granulocyte Macrophage Colony Stimulating Factor, MGC131935, MGC138897, Pluripoietin Alpha,

1mg/ml (determined by Bradford assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Trapnell BC, et al. (2002) Annu Rev Physiol. 64, 775-80;; Wong GG, et al (1985) Science, 228, 810-815;;

> 95% by SDS-PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 50pg/ml.

SDS-PAGE, Bioactivity

Human

Hormones & Cytokines

14.6 kDa (128aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Human GM-CSF in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 50pg/ml.

MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE

Non-Tagged

E.coli

Recombinant Protein

P04141

GM-CSF, Granulocyte-macrophage colony stimulating factor, CSF2, GMCSF, GM-CSF, GM-CSF, Colony-stimulating factor, CSF, Sargramostim, Molgramostin, Granulocyte-macrophage colony-stimulating factor, Granulocyte-macrophage colony stimulating factor Burst Promoting Activity, CMCSF, Colony stimulating factor, Colony Stimulating Factor 2, CSF 2, CSF Alpha, Eosinophil Colony Stimulating Factor,Granulocyte Macrophage Colony Stimulating Factor, MGC131935, MGC138897, Pluripoietin Alpha,MSF0501-10ug,MSF0501-20ug,MSF0501-50ug,MSF0501-100ug,MSF0501-250ug,MSF0501-500ug,MSF0501-1mg,MSF0501-10,MSF0501-20,MSF0501-50,MSF0501-100,MSF0501-250,MSF0501-500,MSF0501-1

Poudel B, et al. Collagen I enhances functional activities of human monocyte-derived dendritic cells via discoidin domain receptor 2. (Cell Immunol. 2012){https://pubmed.ncbi.nlm.nih.gov/23121981/};;Kusakabe Y, et al. A standardized blood test for the routine clinical diagnosis of impaired GM-CSF signaling using flow cytometry. (J Immunol Methods. 2014){https://www.ncbi.nlm.nih.gov/pubmed/25068538}