protein

Recombinant human MIF protein

MIF0501

20 ug

55 USD

Recombinant human MIF protein

1-115aa

MIF0501

NP_002406.1

The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.

Macrophage migration inhibitory factor, Macrophage migration inhibitory factor, GLIF, MMIF, MIF (1-114aa), MIF, EC 5.3.2.1, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, Macrophage migration inhibitory factor Glycosylation inhibiting factor, MIF protein,

1mg/ml (determined by Bradford assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Weiser WY., et al (1989) Proc Natl Acad Sci. 86: 7522-26.;; Bernhagen J., et al (1994) Biochemistry. 33: 14144-55.;; Bucala R., et al. (1996) FASEB J 10: 1607-13.;;

> 95% by SDS-PAGE

SDS-PAGE

Human

Hormones & Cytokines

17 kDa (155aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA

His-Tag

E.coli

Recombinant Protein

P14174

MIF, Macrophage migration inhibitory factor, Macrophage migration inhibitory factor, GLIF, MMIF, MIF (1-114aa), MIF, EC 5.3.2.1, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, Macrophage migration inhibitory factor Glycosylation inhibiting factor, MIF protein,MIF0501-10ug,MIF0501-20ug,MIF0501-50ug,MIF0501-100ug,MIF0501-250ug,MIF0501-500ug,MIF0501-1mg,MIF0501-10,MIF0501-20,MIF0501-50,MIF0501-100,MIF0501-250,MIF0501-500,MIF0501-1

Tsuji S, et al. Effective isolation of RNA aptamer through suppression of PCR bias. (Biochem Biophys Res Commun. 2009){https://pubmed.ncbi.nlm.nih.gov/19520057/};;Tsuji S, et al. RNA aptamer binding to polyhistidine-tag. (Biochem Biophys Res Commun. 2009){https://pubmed.ncbi.nlm.nih.gov/19520059/};;Garai J, et al. Tetralone derivatives are MIF tautomerase inhibitors and attenuate macrophage activation and amplify the hypothermic response in endotoxemic mice. (J Enzyme Inhib Med Chem. 2021){https://pubmed.ncbi.nlm.nih.gov/34225560/};;Garai J, et al. Synthesis and evaluation of a new class of MIF-inhibitors in activated macrophage cells and in experimental septic shock in mice. (Eur J Med Chem. 2023){https://pubmed.ncbi.nlm.nih.gov/36587420/}