protein

Recombinant human Leptin/OB protein

LEP3002

20 ug

55 USD

Recombinant human Leptin/OB protein

22-167aa

LEP3002

NP_000221

Human leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. The function of leptin in regulating appetite and metabolism, as well as the possibility of using leptin as a therapeutic agent, are currently under intense investigation. Recombinant human leptin was overexpressed as insoluble protein aggregate in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

LEP, OB, OBS, Leptin, Obesity factor, Obese protein, Leptin, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, Obesity, Obesity homolog mouse, Obesity Murine Homolog Leptin,

1mg/ml (determined by Bradford assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Jeong KJ., et al. (1999) Appl Environ Microbiol. 65(7), 3027-32.;; Anini Y., et al. (2003) Diabetes. 52(2), 252-259.;; Hyogo H., et al. (2002) J. Biol. Chem. 277(37), 34117-34124.;;

> 95% by SDS-PAGE

SDS-PAGE

Human

Hormones & Cytokines

16 kDa (147aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC

Non-Tagged

E.coli

Recombinant Protein

P41159

Leptin, OB, LEP, OB, OBS, Leptin, Obesity factor, Obese protein, Leptin, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, Obesity, Obesity homolog mouse, Obesity Murine Homolog Leptin,LEP3002-10ug,LEP3002-20ug,LEP3002-50ug,LEP3002-100ug,LEP3002-250ug,LEP3002-500ug,LEP3002-1mg,LEP3002-10,LEP3002-20,LEP3002-50,LEP3002-100,LEP3002-250,LEP3002-500,LEP3002-1

Lee EN, et al. Stabilizing peptide fusion for solving the stability and solubility problems of therapeutic proteins. Pharm Res. 2005 Oct;22(10):1735-46.{https://pubmed.ncbi.nlm.nih.gov/16180132/}