protein

Recombinant human IL-4 protein

ILD0905

10 ug

160 USD

Recombinant human IL-4 protein

25-153aa

ILD0905

NP_000580

Interleukin-4 (IL-4) is a pleiotropic cytokine that has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of naive helper T-cell to Th2 cells. Interleukin-4 is a co-stimulator of DNA-synthesis and is induces the expression of class II MHC molecules on resting B-cells. It also enhances both secretion and cell surface expression of IgE and IgG1. Recombinant Interleukin-4 protein, fused to His-tag at N-terminus, was expressed in E. coli and was purified by conventional chromatography techniques, after refolding of the isolated inclusion bodies in a renaturation buffer.

Pitrakinra, MGC79402, Lymphocyte stimulatory factor 1, Interleukin-4 isoform 1, Interleukin 4, IL-4, BSF1, Binetrakin, BCGF1, B-cell stimulatory factor 1, B-cell growth factor 1, BCDF

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Van Kimmenade A., et al. (1988) Eur J Biochem. 173(1):109-14.;; Yokota T., et al. (1986) Proc. Natl. Acad. Sci. uSA. 83(16):5894-8.;;

> 95% by SDS-PAGE

The ED50 for this effect is less or equal to 0.5ng/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.

SDS-PAGE, Bioactivity

Human

Hormones & Cytokines

17.2 kDa (150aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

The ED50 for this effect is less or equal to 0.5 ng/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.

MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS

His-Tag

E.coli

Recombinant Protein

P05112

Pitrakinra, Pitrakinara, MGC79402, Lymphocyte stimulatory factor 1, Interleukin-4 isoform 1, Interleukin-4, Interleukin4, Interleukin 4 isoform 1, Interleukin 4, IL-4, IL4, IL 4, BSF-1, BSF1, BSF 1, Binetrakin, BCGF-1, BCGF1, BCGF 1, B-cell stimulatory factor 1, B-cell growth factor 1, BCDF, B cell stimulatory factor 1, B cell growth factor 1, ILD0905-10ug, ILD0905-20ug, ILD0905-50ug, ILD0905-100ug, ILD0905-250ug, ILD0905-500ug, ILD0905-1mg, ILD0905-010, ILD0905-020, ILD0905-050, ILD0905-100, ILD0905-250, ILD0905-500, ILD0905-01M

Lee HM, et al. A comparative study of the effects of inhibitory cytokines on human natural killer cells and the mechanistic features of transforming growth factor-beta. (Cell Immunol. 2014){https://pubmed.ncbi.nlm.nih.gov/24879062/}