Recombinant human IL-3 protein | NKMAX ILC0702 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human IL-3 protein

catalog :

ILC0702

quantity :

20 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human IL-3 protein

Domain :

20-152aa

Catalog number (SKU) :

ILC0702

NCBI :

NP_000579

Description :

Interleukin-3 (IL-3) stimulates the proliferation of hematopoietic pluripotent progenitor cells. It is secreted by activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response. IL-3 exerts its biological activities through binding to specific cell surface receptors. IL-3 receptor is a complex consisting of alpha and beta subunits, which in turn induces activation of JAK2/ STAT5, induction of c-myc, and activation of the Ras pathway. Human IL3 is a protein of 15-17 kDa (133 amino acids). It is synthesized as a precursor containing a hydrophobic secretory signal sequence of 19 amino acids. IL3 contains two putative glycosylation sites at positions 15 and 70 and contains a single disulfide bond (Cys16/84). Recombinant human IL-3 was expressed in E. coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.

Alternative Names :

IL-3, Multipotential colony-stimulating factor(multi-CSF), Mast cell growth factor(MCGF), interleukin 3 (colony-stimulating factor, multiple), MuLTI-CSF, MCGF, multilineage-colony-stimulating factor, hematopoietic growth factor, P-cell stimulating factor, mast-cell growth factor

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol, 0.2mM PMSF

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Allan SM., et al. (2005), Nat. Rev. Immunol. 5:629.;; Michaud DS., et al. (2006), Cancer Res. 66(8):4525-30;;

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effects less or equal to 0.15ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Hormones & Cytokines

Molecular Weight :

17.3 kDa (154aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human IL-3 stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range is less or equal 0.15 ng/ml.

Amino Acid Sequence :

APMTQTTSL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P08700

Internal Search Terms :

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more info or order :

NKMAX product webpage