protein

Recombinant human IL-33 protein

ILC0701

10 ug

100 USD

Recombinant human IL-33 protein

112-270aa

ILC0701

NP_254274

Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine that may also regulate gene transcription in producer cells. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1. Recombinant human IL-33(C-terminal fragment) was expressed in E.coli and purified by using conventional chromatography techniques.

Interleukin-33, Interleukin-1 family member 11, IL-1F11, Nuclear factor from high endothelial venules, NF-HEV, DVS27-related protein, DVS27, C9orf26

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0)

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Schmitz., et al. (2005) immunity 23(5):479-90;; Baekkevold, E.S., et al. (2003) Am. J.Pathol. 163(1):69-90;;

> 95% by SDS-PAGE

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.3ng/ml.

SDS-PAGE, Bioactivity

Human

Hormones & Cytokines

18.1 kDa (160aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Human IL-33 in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.3ng/ml.

MSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET

Non-Tagged

E.coli

Recombinant Protein

O95760

IL33, Interleukin 33, DVS27, NF-HEV, NKHEV, C9orf26, IL-33, Interleukin 33 CHROMOSOME 9 OPEN READING FRAME 26, DKFZp586H0523, DVS27 related protein, IL 1F11, IL 33, IL1F11, Interleukin 1 family member 11, INTERLEuKIN 33 NFHEV, Interleukin 33 precursor, Interleukin33, NF HEV, NFHEV, Nuclear factor from high endothelial venules, RP11 575C20.2, il33,ILC0701-10ug,ILC0701-20ug,ILC0701-50ug,ILC0701-100ug,ILC0701-250ug,ILC0701-500ug,ILC0701-1mg,ILC0701-10,ILC0701-20,ILC0701-50,ILC0701-100,ILC0701-250,ILC0701-500,ILC0701-1

Ito R, et al. A humanized mouse model to study asthmatic airway inflammation via the human IL-33/IL-13 axis. (JCI Insight. 2018){https://pubmed.ncbi.nlm.nih.gov/30385714/}