Recombinant human IL-1 beta/IL1B protein | NKMAX ILB0701 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human IL-1 beta/IL1B protein

catalog :

ILB0701

quantity :

10 ug

price :

44 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human IL-1 beta/IL1B protein

Domain :

117-269aa

Catalog number (SKU) :

ILB0701

NCBI :

NP_000567.1

Description :

Interleukin-1 (IL-1) has been considered a potentially important inflammatory mediator. It is composed of two distinct proteins, called as IL-1alpha and IL-1beta. IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta bind to the same receptor and they are structurally related polypeptides that share approximately 20% amino acid. Recombinant human IL-1beta was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Interleukin-1 beta, IL-1 beta, Catabolin, IL1F2

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Allan SM., et al. (2005), Nat. Rev. Immunol. 5:629.;; Michaud DS., et al. (2006), Cancer Res.66(8):4525-30;;

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 12pg/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Hormones & Cytokines

Molecular Weight :

17 kDa (154aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human IL-1 beta /IL-1F2 stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range ≤ 12 pg/ml.

Amino Acid Sequence :

MAPVRSLNCT LRDSQQKSLV MSGPYELKAL HLQGQDMEQQ VVFSMSFVQG EESNDKIPVA LGLKEKNLYL SCVLKDDKPT LQLESVDPKN YPKKKMEKRF VFNKIEINNK LEFESAQFPN WYISTSQAEN MPVFLGGTKG GQDITDFTMQ FVSS

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P01584

Internal Search Terms :

IL1 b, IL1F2,ILB0701-10ug,ILB0701-20ug,ILB0701-50ug,ILB0701-100ug,ILB0701-250ug,ILB0701-500ug,ILB0701-1mg,ILB0701-10,ILB0701-20,ILB0701-50,ILB0701-100,ILB0701-250,ILB0701-500,ILB0701-1

References with this Product :

Choi DY, et al. Molecularly imprinted polymer-based electrochemical impedimetric sensors on screen-printed carbon electrodes for the detection of trace cytokine IL-1β. (Biosens Bioelectron. 2022){https://pubmed.ncbi.nlm.nih.gov/35144170/};;Park RW, et al. Mobile Point-of-Care Device Using Molecularly Imprinted Polymer-Based Chemosensors Targeting Interleukin-1β Biomarker. (Biosensors. 2023){https://pubmed.ncbi.nlm.nih.gov/38131773/}

more info or order :

NKMAX product webpage