protein

Recombinant human IL-2 (C145S) protein

ILB0501

20 ug

105 USD

Recombinant human IL-2 (C145S) protein

22-153aa

ILB0501

NP_000577.2

IL-2, also known as T cell growth factor (TCGF), is an immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. This recombinant human IL-2 is a 15. 4 kDa protein, containing 133 amino acid residues including one intra chain disulfide bond. Recombinant human IL-2 was overexpressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

IL-2, TCGF, Aldesleukin, T-cell growth factor, Interleukin-2

0.5mg/ml (determined by Bradford assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Morgan DA., et al. (1976). Science, 193. 1007-8. ;; Watson J., et al. (1980). Immunol Rev, 51. 257-78.;; Taniguchi T., et al. (1983). Nature, 302. 305-10.;;

> 95% by SDS-PAGE

Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 range ≤0.65ng/ml.

SDS-PAGE, Bioactivity

Human

Hormones & Cytokines

15.4 kDa (133aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Human IL-2 (C145S) stimulates cell proliferation of the CTLL2 mouse cytotoxic T cells. The ED50 range ≤0.65 ng/ml.

MPTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFSQSIIS TLT

Non-Tagged

E.coli

Recombinant Protein

P60568

IL2 (C145S),ILB0501-10ug,ILB0501-20ug,ILB0501-50ug,ILB0501-100ug,ILB0501-250ug,ILB0501-500ug,ILB0501-1mg,ILB0501-10,ILB0501-20,ILB0501-50,ILB0501-100,ILB0501-250,ILB0501-500,ILB0501-1

Kim HR, et al. Anti-cancer activity and mechanistic features of a NK cell activating molecule. (Cancer Immunol Immunother. 2009){https://pubmed.ncbi.nlm.nih.gov/19259669/};;Koh J, et al. Susceptibility of CD24(+) ovarian cancer cells to anti-cancer drugs and natural killer cells. 9Biochem Biophys Res Commun. 2012){https://pubmed.ncbi.nlm.nih.gov/22995296/};;Lee HM, et al. A comparative study of the effects of inhibitory cytokines on human natural killer cells and the mechanistic features of transforming growth factor-beta. (Cell Immunol. 2014){https://pubmed.ncbi.nlm.nih.gov/24879062/};;Shin HW, et al. Role of c-Myb in the regulation of natural killer cell activity. (Biochem Biophys Res Commun. 2018){https://pubmed.ncbi.nlm.nih.gov/30103947/};;Lee Y, et al. In vivo Anti-Cancer Effects of Resveratrol Mediated by NK Cell Activation. (J Innate Immun. 2020){https://pubmed.ncbi.nlm.nih.gov/32937636/};;Lee YJ, et al. Resveratrol Activates Natural Killer Cells through Akt- and mTORC2-Mediated c-Myb Upregulation. (Int J Mol Sci. 2020){https://pubmed.ncbi.nlm.nih.gov/33339133/};;Ko E, et al. ADSC secretome constrains NK cell activity by attenuating IL-2-mediated JAK-STAT and AKT signaling pathway via upregulation of CIS and DUSP4. (Stem Cell Res Ther. 2023){https://pubmed.ncbi.nlm.nih.gov/37964351/}