protein

Recombinant human IGF-1 protein

IGF0501

20 ug

30 USD

Recombinant human IGF-1 protein

49-118aa

IGF0501

NP_000609.1

The Insulin-like growth factor-1 (IGF-1) is mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGF-1 is predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGF-1 belongs to the insulin gene family, which also contains insulin and relaxin. The IGF-1 is similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. This recombinant human IGF-I is globular protein containing 70 amino acids, and 3 intra-molecular disulfide bonds. Recombinant IGF-1 was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

IBP1, IGF-1, IGFI, IGF-IA, IGF-IB, Insulin like growth factor 1 (somatomedin C), Insulin like growth factor IA, Insulin like growth factor IB, Insulin-like growth factor-1 IGF IA, Mechano growth factor, MGF, Somatomedin C

1mg/ml (determined by Bradford assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Rinderknecht E., et al (1978) J Biol.Chem, 253: 2769. ;; Zumstein P., et al (1987) J Biol. Chem, 262: 11252.;; Rabinovsky ED (2004) Neurol Res, 26: 204-210;;

> 95% by SDS-PAGE

Measured in a cell proliferation assay using MCF-7 human breast cancer cells. The ED50 range ≤ 5ng/ml.

SDS-PAGE, Bioactivity

Human

Hormones & Cytokines

7.7 kDa (71aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Human IGF-I stimulates cell proliferation of the MCF-7 human breast cancer cells. The ED50 range ≤ 5 ng/ml.

MGPETLCGAE LVDALQFVCG DRGFYFNKPT GYGSSSRRAP QTGIVDECCF RSCDLRRLEM YCAPLKPAKS A

Non-Tagged

E.coli

Recombinant Protein

P05019

IBP1, IGF-IB, IGF IB, IGF1, IGF-1, IGFI, IGF-I, IGFIA, IGF-IA, Insulin like growth factor 1, Insulin like growth factor 1 (somatomedin C), Insulin like growth factor IA, Insulin like growth factor IB, Insulin-like growth factor-1, Insulin-like growth factor-1 IGF IA, Mechano growth factor, MGF, Somatomedin C, Somatomedin-C, IGF0501-10ug, IGF0501-20ug, IGF0501-50ug, IGF0501-100ug, IGF0501-250ug, IGF0501-500ug, IGF0501-1mg, IGF0501-010, IGF0501-020, IGF0501-050, IGF0501-100, IGF0501-250, IGF0501-500, IGF0501-01M

Shin WJ, Kim JS. The Effects of Recombinant Synucleins and Insulin-like Growth Factor 1 on Cancer Cell Migration. (Journal of Bacteriology and Virology 2012){https://doi.org/10.4167/jbv.2012.42.2.133}