Recombinant human IFN-gamma/IFNG protein | NKMAX IFG4001 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human IFN-gamma/IFNG protein

catalog :

IFG4001

quantity :

20 ug

price :

55 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human IFN-gamma/IFNG protein

Domain :

24-166aa

Catalog number (SKU) :

IFG4001

NCBI :

NP_000610.2

Description :

Nature human interferon-gamma is composed of 143amino acid residues without cysteine residues and is glycosylated. Recombinant human interferon-gamma was expressed in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Additional amino acid (Methionine) is attached at N-terminus.

Alternative Names :

Interferon gamma, Immune interferon, IFG, IFI, IMD69

Concentration :

1mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Sen GC, Lengyel P (1992) J. Biol. Chem. 267(8):5017-5020;; Tsutomu Arakawa, Yeuh-Rong Hsu (1987) Biochemistry. 26:5428-5432;; Ernst Rinderknecht, et al (1984) J. Biol. Chem. 259(11):6790-6797;;

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cytotoxicity assay using WiDr human colon colorectal adenocarcinoma cells. The ED50 range ≤ 1.5ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Hormones & Cytokines

Molecular Weight :

16.9 kDa (144aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human Interferon-gamma stimulates cytotoxicity of the WiDr human colon colorectal adenocarcinoma cells. The ED50 range ≤ 1.5ng/ml

Amino Acid Sequence :

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRGR RASQ

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P01579

Internal Search Terms :

Interferon g, Ifng, Interferon-g, IFN-gamma, Interferon gamma, IFNG, IFG, IFI, IFN gamma, IFN immune, Immune interferon, Interferon gamma precursor, Macrophage activating factor, MAF, T Cell interferon, Type II Interferon,IFG4001-10ug,IFG4001-20ug,IFG4001-50ug,IFG4001-100ug,IFG4001-250ug,IFG4001-500ug,IFG4001-1mg,IFG4001-10,IFG4001-20,IFG4001-50,IFG4001-100,IFG4001-250,IFG4001-500,IFG4001-1

References with this Product :

Thao NP, et al. NF-κB inhibitory activity of polyoxygenated steroids from the Vietnamese soft coral Sarcophyton pauciplicatum. (Bioorg Med Chem Lett. 2014){https://pubmed.ncbi.nlm.nih.gov/24852121/}

more info or order :

NKMAX product webpage