protein

Recombinant human Hexokinase 1 protein

HXK0704

20 ug

160 USD

Recombinant human Hexokinase 1 protein

1-917aa

HXK0704

NP_000179.2

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have been associated with hemolytic anemia due to hexokinase deficiency. Recombinant human Hexokinase1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

HK1, Hexokinase 1, EC 2.7.1.1, Hexokinase type I, HK I, Brain form hexokinase, Hexokinase-1, BB404130, dea, Glycolytic enzyme, HEXOKIN, Hexokinase PI, Hexokinase type I, Hexokinase, tumor isozyme, Hexokinase-A, HK1 tb, Hk1-s, HK1-ta, HK1-tc, HKI, HXK1, mHk1-s.

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Jon E. et al.,(2003) J.Exp Biology. 206 : 2049-2057.;; Furuta H. et al.,(1996) Genomics. 36(1):206-9.;;

> 85% by SDS-PAGE

Specific activity is > 8,000pmol/min/ug. One unit will convert 1pmole of D-Glucose to D-Glucose-6-phosphate per minute at pH 7.5 at 37C.

SDS-PAGE, Enzyme Activity

Human

Signal Transduction

104.6 kDa (937aa)

Specific activity is > 8,000 pmol/min/ug. One unit will convert 1 pmole of D-Glucose to D-Glucose-6-phosphate per minute at pH 7.5 at 37C.

MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLIDIM TRFRKEMKNG LSRDFNPTAT VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE KNQNVHMESE VYDTPENIVH GSGSQLFDHV AECLGDFMEK RKIKDKKLPV GFTFSFPCQQ SKIDEAILIT WTKRFKASGV EGADVVKLLN KAIKKRGDYD ANIVAVVNDT VGTMMTCGYD DQHCEVGLII GTGTNACYME ELRHIDLVEG DEGRMCINTE WGAFGDDGSL EDIRTEFDRE IDRGSLNPGK QLFEKMVSGM YLGELVRLIL VKMAKEGLLF EGRITPELLT RGKFNTSDVS AIEKNKEGLH NAKEILTRLG VEPSDDDCVS VQHVCTIVSF RSANLVAATL GAILNRLRDN KGTPRLRTTV GVDGSLYKTH PQYSRRFHKT LRRLVPDSDV RFLLSESGSG KGAAMVTAVA YRLAEQHRQI EETLAHFHLT KDMLLEVKKR MRAEMELGLR KQTHNNAVVK MLPSFVRRTP DGTENGDFLA LDLGGTNFRV LLVKIRSGKK RTVEMHNKIY AIPIEIMQGT GEELFDHIVS CISDFLDYMG IKGPRMPLGF TFSFPCQQTS LDAGILITWT KGFKATDCVG HDVVTLLRDA IKRREEFDLD VVAVVNDTVG TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMVEGDQ GQMCINMEWG AFGDNGCLDD IRTHYDRLVD EYSLNAGKQR YEKMISGMYL GEIVRNILID FTKKGFLFRG QISETLKTRG IFETKFLSQI ESDRLALLQV RAILQQLGLN STCDDSILVK TVCGVVSRRA AQLCGAGMAA VVDKIRENRG LDRLNVTVGV DGTLYKLHPH FSRIMHQTVK ELSPKCNVSF LLSEDGSGKG AALITAVGVR LRTEASS

His-Tag

E.coli

Recombinant Protein

P19367

Hexokinase1,HXK0704-10ug,HXK0704-20ug,HXK0704-50ug,HXK0704-100ug,HXK0704-250ug,HXK0704-500ug,HXK0704-1mg,HXK0704-10,HXK0704-20,HXK0704-50,HXK0704-100,HXK0704-250,HXK0704-500,HXK0704-1

Tsumura Y, et al. TMG-123, a novel glucokinase activator, exerts durable effects on hyperglycemia without increasing triglyceride in diabetic animal models. (PLoS One. 2017){https://pubmed.ncbi.nlm.nih.gov/28207836/};;Yoshitake T, et al. Anti-Hexokinase 1 Antibody as a Novel Serum Biomarker of a Subgroup of Diabetic Macular Edema. (Sci Rep. 2019){https://pubmed.ncbi.nlm.nih.gov/30886155/}