protein

Recombinant human Hexokinase 4 protein

HXK0702

20 ug

160 USD

Recombinant human Hexokinase 4 protein

1-465aa

HXK0702

NP_000153

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase4 is found in the liver and pancreatic beta-cells, where it is controlled by insulin (activation) and glucagon (inhibition). In pancreatic beta-cells, type IV enzyme acts as a glucose sensor to modify insulin secretion. Hexokinase4 is commonly called glucokinase. Recombinant human Hexokinase4, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

GCK, glucokinase, MODY2, Maturity onset diabetes of the young 2, Hexokinase 4, HK4, Hexokinase type IV, HK IV, Hexokinase-D

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Jon E. et al.,(2003) J.Exp Biology. 206: 2049-2057

> 95% by SDS-PAGE

SDS-PAGE

Human

Signal Transduction

54.3 kDa (485aa)

MGSSHHHHHH SSGLVPRGSH MLDDRARMEA AKKEKVEQIL AEFQLQEEDL KKVMRRMQKE MDRGLRLETH EEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEEGQWS VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF SFPVRHEDID KGILLNWTKG FKASGAEGNN VVGLLRDAIK RRGDFEMDVV AMVNDTVATM ISCYYEDHQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR MCVNTEWGAF GDSGELDEFL LEYDRLVDES SANPGQQLYE KLIGGKYMGE LVRLVLLRLV DENLLFHGEA SEQLRTRGAF ETRFVSQVES DTGDRKQIYN ILSTLGLRPS TTDCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED VMRITVGVDG SVYKLHPSFK ERFHASVRRL TPSCEITFIE SEEGSGRGAA LVSAVACKKA CMLGQ

His-Tag

E.coli

Recombinant Protein

P35557

Glucokinase, GCK, GCK, Hexokinase 4, EC 2.7.1.2, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D, Glucokinase, ATP D hexose 6 phosphotransferase, GK, GLK, Glucokinase, Glucokinase isoform 1, Hexokinase 4, Hexokinase D, Hexokinase D pancreatic isozyme, Hexokinase4, HexokinaseD, HHF 3, HHF3, HK 4, HXKP, Maturity onset diabetes of the young 2, MODY 2, MODY2.,HXK0702-10ug,HXK0702-20ug,HXK0702-50ug,HXK0702-100ug,HXK0702-250ug,HXK0702-500ug,HXK0702-1mg,HXK0702-10,HXK0702-20,HXK0702-50,HXK0702-100,HXK0702-250,HXK0702-500,HXK0702-1