protein

Recombinant human HSP10/EPF protein

HSP0801

20 ug

80 USD

Recombinant human HSP10/EPF protein

1-102aa

HSP0801

NP_002148

Heat shock protein (HSP) 10 is a member of the highly conserved group of molecular chaperons, which are necessary for efficient folding of many proteins in normal and stress conditions and have been implicated in several human diseases. HSP10 is the ~10kD mammalian equivalent to GroES of E. coli. The function of Hsp10 is to bind Hsp60 (also known as chaperonin-60), in the presence of ATP, thereby promoting a conformational change in Hsp60 and enclosing the protein substrate within the complex. ATP hydrolysis by Hsp60 destabilizes the Hsp60/ Hsp10 complex, allowing it to dissociate and release the substrate protein. Recombinant human HSP10 was expressed in E. coli and purified by using conventional chromatography techniques.

Heat shock protein 10, Chaperonin 10, CPN10, GroES, Heat shock protein 10, HSP10, Heat shock protein 10 10 kDa chaperonin, HSPE 1, HSPE1, 10 kDa heat shock protein mitochondrial, Cpn 10, Early pregnancy factor, EPF, Heat shock 10kD protein 1, Heat shock 10kD protein 1 chaperonin 10, Heat shock 10kDa protein 1, HSP 10, HSP10, Heat shock 10kDa protein 1 chaperonin 10,

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 50mM NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Lin KM., et al. (2004), FASEB J. 18(9):1004-6;; Hohfeld J., et al. (1994), J. Cell BIol. 126(2):305-15

> 95% by SDS-PAGE

SDS-PAGE

Human

Heat Shock Proteins

10.9 kDa (102aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

MAGQAFRKFL PLFDRVLVER SAAETVTKGG IMLPEKSQGK VLQATVVAVG SGSKGKGGEI QPVSVKVGDK VLLPEYGGTK VVLDDKDYFL FRDGDILGKY VD

Non-Tagged

E.coli

Recombinant Protein

P61604

HSP10, EPF, Heat shock protein 10, Chaperonin 10, CPN10, GroES, Heat shock protein 10, HSP10, Heat shock protein 10 10 kDa chaperonin, HSPE 1, HSPE1, 10 kDa heat shock protein mitochondrial, Cpn 10, Early pregnancy factor, EPF, Heat shock 10kD protein 1, Heat shock 10kD protein 1 chaperonin 10, Heat shock 10kDa protein 1, HSP 10, HSP10, Heat shock 10kDa protein 1 chaperonin 10,HSP0801-10ug,HSP0801-20ug,HSP0801-50ug,HSP0801-100ug,HSP0801-250ug,HSP0801-500ug,HSP0801-1mg,HSP0801-10,HSP0801-20,HSP0801-50,HSP0801-100,HSP0801-250,HSP0801-500,HSP0801-1

Kaiser F, et al. Monocyte cytokine synthesis in response to extracellular cell stress proteins suggests these proteins exhibit network behaviour. (Cell Stress Chaperones. 2014){https://pubmed.ncbi.nlm.nih.gov/23775284/}