protein

Recombinant human HSP70/HSPA1A protein

HSP0603

20 ug

55 USD

Recombinant human HSP70/HSPA1A protein

1-641aa

HSP0603

NP_005336.3

Hsp70 is a human heat shock protein. Hsp70 is an important part of the cell's machinery for protein folding, and help to protect cells from stress. In most species, there are many proteins that belong to the hsp70 family. Some of these are only expressed under stress conditions, while some are present in cells under normal growth conditions and are not heat-inducible. They can be found in different cellular compartments (nuclear, cytosolic, mitochondrial, endoplasmic reticulum, etc. ). Recombinant human Hsp70, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Heat shock 70kDa protein 1A, Heat shock protein family A member 1A, HSP70, HSP70.1, HSP70-1/HSP70-2, HSPA1, HSPA1A, HSPA1B

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 2mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Gething M-J, et al. (1992). Nature. 355. 33-45;; Lewis MJ, et al. EMBO J. (1985) 4:3137-3143

> 95% by SDS-PAGE

SDS-PAGE

Human

Heat Shock Proteins

72.2 kDa (661aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

MAKAAAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFQVINDGDK PKVQVSYKGD TKAFYPEEIS SMVLTKMKEI AEAYLGYPVT NAVITVPAYF NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVNH FVEEFKRKHK KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS TRIPKVQKLL QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS LGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT KDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK ITITNDKGRL SKEEIERMVQ EAEKYKAEDE VQRERVSAKN ALESYAFNMK SAVEDEGLKG KISEADKKKV LDKCQEVISW LDANTLAEKD EFEHKRKELE QVCNPIISGL YQGAGGPGPG GFGAQGPKGG SGSGPTIEEV D

His-Tag

E.coli

Recombinant Protein

P0DMV8

Heat shock 70 kDa protein 1A, Heat shock 70kDa protein 1A, Heat shock protein family A member 1A, HSP 70, HSP70, HSP70.1, HSP70-1/HSP70-2, HSPA1, HSPA1A, HSPA1B, HSP0603-10ug, HSP0603-20ug, HSP0603-50ug, HSP0603-100ug, HSP0603-250ug, HSP0603-500ug, HSP0603-1mg, HSP0603-010, HSP0603-020, HSP0603-050, HSP0603-100, HSP0603-250, HSP0603-500, HSP0603-01M

Kawakami F, et al. Stimulatory effect of α-synuclein on the tau-phosphorylation by GSK-3β. (FEBS J. 2011){https://pubmed.ncbi.nlm.nih.gov/21985244/};;Roy A, et al. Mast Cell Chymase Degrades the Alarmins Heat Shock Protein 70, Biglycan, HMGB1, and Interleukin-33 (IL-33) and Limits Danger-induced Inflammation. (J Biol Chem. 2014){https://www.ncbi.nlm.nih.gov/pubmed/24257755};;Kaiser F, et al. Monocyte cytokine synthesis in response to extracellular cell stress proteins suggests these proteins exhibit network behaviour. (Cell Stress Chaperones. 2014){https://pubmed.ncbi.nlm.nih.gov/23775284/}