protein

Recombinant human HSP27 protein

HSP0503

20 ug

55 USD

Recombinant human HSP27 protein

1-205aa

HSP0503

NP_001531

Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38). Recombinant human Hsp27 was overexpressed in E. coli and purified by conventional chromatography.

Heat shock 27 kDa protein, Hspb1, Hsp25, Hsp27, Hsp27, HspB1, Heat shock 27 kDa protein, HSP 27, Growth-related 25 kDa protein, P25, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein 28 kDa heat shock protein, CMT2F, DKFZp586P1322, Estrogen regulated 24 kDa protein, at shock 25kDa protein 1, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat Shock Protein 27, Heat shock protein beta 1, Heat Shock Protein27, HS.76067, Hsp 28, Hsp B1, Hsp28, HspB1, SRP27, Stress responsive protein 27.

1mg/ml (determined by Bradford assay)

Liquid in. 20mM HEPES buffer (pH 7.4) containing 100mM KCl, 1mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Ehrnsperger M.,et al. (1997) EMBO J. 16, 221-229;; Butt E., et al. (2001) J. Biol. Chem. 276, 7108-7113;;

> 95% by SDS-PAGE

SDS-PAGE

Human

Heat Shock Proteins

22.7 kDa (205aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK

Non-Tagged

E.coli

Recombinant Protein

P04792

HSP27, Heat shock 27 kDa protein, Hspb1, Hsp25, Hsp27, Hsp27, HspB1, Heat shock 27 kDa protein, HSP 27, Growth-related 25 kDa protein, P25, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein 28 kDa heat shock protein, CMT2F, DKFZp586P1322, Estrogen regulated 24 kDa protein, at shock 25kDa protein 1, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat Shock Protein 27, Heat shock protein beta 1, Heat Shock Protein27, HS.76067, Hsp 28, Hsp B1, Hsp28, HspB1, SRP27, Stress responsive protein 27.,HSP0503-10ug,HSP0503-20ug,HSP0503-50ug,HSP0503-100ug,HSP0503-250ug,HSP0503-500ug,HSP0503-1mg,HSP0503-10,HSP0503-20,HSP0503-50,HSP0503-100,HSP0503-250,HSP0503-500,HSP0503-1

Kaiser F, et al. Monocyte cytokine synthesis in response to extracellular cell stress proteins suggests these proteins exhibit network behaviour. (Cell Stress Chaperones. 2014){https://pubmed.ncbi.nlm.nih.gov/23775284/};;Casola C, et al. S100 alone has the same destructive effect on retinal ganglion cells as in combination with HSP 27 in an autoimmune glaucoma model. (J Mol Neurosci. 2015){https://pubmed.ncbi.nlm.nih.gov/25577368/};;Casola C, et al. Specific Inner Retinal Layer Cell Damage in an Autoimmune Glaucoma Model Is Induced by GDNF With or Without HSP27. (Invest Ophthalmol Vis Sci. 2016) {https://pubmed.ncbi.nlm.nih.gov/27391553/};;Reinehr S, et al. HSP27 immunization reinforces AII amacrine cell and synapse damage induced by S100 in an autoimmune glaucoma model. (Cell Tissue Res. 2018){https://pubmed.ncbi.nlm.nih.gov/29064077/};;Kaiser F, et al. Association between circulating levels of heat-shock protein 27 and aggressive periodontitis. (Cell Stress Chaperones. 2018){https://pubmed.ncbi.nlm.nih.gov/29766408/};;Grotegut P, et al. Destructive Effect of Intravitreal Heat Shock Protein 27 Application on Retinal Ganglion Cells and Neurofilament. (Int J Mol Sci. 2020){https://pubmed.ncbi.nlm.nih.gov/31952234/};;Eickholz P, et al. Effect of nonsurgical periodontal therapy on haematological parameters in grades B and C periodontitis: an exploratory analysis. (Clin Oral Investig. 2020){https://pubmed.ncbi.nlm.nih.gov/32385656/};;Grotegut P, et al. Heat Shock Protein 27 Injection Leads to Caspase Activation in the Visual Pathway and Retinal T-Cell Response. (Int J Mol Sci. 2021){https://pubmed.ncbi.nlm.nih.gov/33419223/};;Erb C, et al. HSP27 induced glaucomatous damage in mice of young and advanced age. (Front Cell Neurosci. 2023){https://pubmed.ncbi.nlm.nih.gov/37744880/};;Joachim SC, Reinehr S. Does HSP27 injection induce glaucoma damage in mice? (Neural Regen Res. 2024){https://pubmed.ncbi.nlm.nih.gov/38526268/}