protein

Recombinant human HMG-1/HMGB1 protein

HMG0801

20 ug

105 USD

Recombinant human HMG-1/HMGB1 protein

1-215aa

HMG0801

NP_002119.1

High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor. Recombinant human HMGB1 was expressed in insect cells and purified by conventional chromatography techniques.

Sulfoglucuronyl carbohydrate binding protein, SBP-1, HMGB1, HMG3, HMG1, High-mobility group box1, High-mobility group (nonhistone chromosomal) protein 1, DKFZp686A04236, Amphoterin

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM EDTA, 0.5mM DTT, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Palumbo R J., et al. (2004) Cell Biol. 164(3):441-9.;; Andersson u., et al (2002) Leukoc Biol. 72(6):1084-91.;;

> 90% by SDS-PAGE

SDS-PAGE

Human

Epigenomics (Transcription & Translation)

25 kDa (223aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDE

His-Tag

Baculovirus

Recombinant Protein

P09429

Sulfoglucuronyl carbohydrate binding protein, SBP-1, SBP1, SBP 1, HMGB1, HMGB 1, HMG3, HMG1, HMG 1, High-mobility group box1, High-mobility group (nonhistone chromosomal) protein 1, DKFZp686A04236, Amphoterin, HMG 3, High mobility group box1, High mobility group (nonhistone chromosomal) protein 1, High mobility group protein 1, HMG0801-10ug, HMG0801-20ug, HMG0801-50ug, HMG0801-100ug, HMG0801-250ug, HMG0801-500ug, HMG0801-1mg, HMG0801-010, HMG0801-020, HMG0801-050, HMG0801-100, HMG0801-250, HMG0801-500, HMG0801-01M

Min HJ, et al. Chaperone-like Activity of High-Mobility Group Box 1 Protein and Its Role in Reducing the Formation of Polyglutamine Aggregates. (J Immunol. 2013){https://www.ncbi.nlm.nih.gov/pubmed/23303669};;Slean MM, et al. Interconverting Conformations of Slipped-DNA Junctions Formed by Trinucleotide Repeats Affect Repair Outcome. (Biochemistry. 2013){https://www.ncbi.nlm.nih.gov/pubmed/23339280};;Takahashi T, et al. Bone-Targeting Endogenous Secretory Receptor for Advanced Glycation End Products Rescues Rheumatoid Arthritis. (Mol Med. 2013){https://www.ncbi.nlm.nih.gov/pubmed/23821362};;Roy A, et al. Mast cell chymase degrades the alarmins heat shock protein 70, biglycan, HMGB1, and interleukin-33 (IL-33) and limits danger-induced inflammation. (J Biol Chem. 2014){https://pubmed.ncbi.nlm.nih.gov/24257755/};;Sugimoto K, et al. Receptor for Advanced Glycation End Products - Membrane Type1 Matrix Metalloproteinase Axis Regulates Tissue Factor Expression via RhoA and Rac1 Activation in High-Mobility Group Box-1 Stimulated Endothelial Cells. (PLoS One. 2014){https://pubmed.ncbi.nlm.nih.gov/25490770/};;Hernández-Pando R, et al. The Role of High Mobility Group Box 1 Protein (HMGB1) in the Immunopathology of Experimental Pulmonary Tuberculosis. (PLoS One. 2015){https://pubmed.ncbi.nlm.nih.gov/26201072/};;Ito M, et al. High mobility group box 1 enhances hyperthermia-induced seizures and secondary epilepsy associated with prolonged hyperthermia-induced seizures in developing rats. (Metab Brain Dis. 2017){https://pubmed.ncbi.nlm.nih.gov/28879430/}