Recombinant e.coli GrpE protein (V44I) | NKMAX GRP0701 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant e.coli GrpE protein (V44I)

catalog :

GRP0701

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant e.coli GrpE protein (V44I)

Domain :

1-197aa

Catalog number (SKU) :

GRP0701

NCBI :

NP_417104.1

Description :

GrpE, co-chaperone of E. coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding. Recombinant GrpE protein was overexpressed in E. coli and purified by using the conventional column chromatography techniques.

Alternative Names :

HSP-70 cofactor, Heat shock protein B25.3, HSP24, GrpE, HSP 70 cofactor.

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 100mM NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Lipinska B, King J, Ang D, Georgopoulos C (1988). Nucleic Acids Res.16: 545-7562;; Liberek K., Marszzalek J., Ang D, Georgopoulos C., Zylicz M. (1991). Proc. Natl. Acad.Sci. u.S.A. 88: 2874-2878.;;

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

E.coli

Gene Category :

Heat Shock Proteins

Molecular Weight :

21.8 kDa (197aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P09372

Internal Search Terms :

GrpE, HSP-70 cofactor, Heat shock protein B25.3, HSP24, GrpE, HSP 70 cofactor.,GRP0701-10ug,GRP0701-20ug,GRP0701-50ug,GRP0701-100ug,GRP0701-250ug,GRP0701-500ug,GRP0701-1mg,GRP0701-10,GRP0701-20,GRP0701-50,GRP0701-100,GRP0701-250,GRP0701-500,GRP0701-1

more info or order :

NKMAX product webpage