Recombinant E.coli GroEL protein | NKMAX GRL3001 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant E.coli GroEL protein

catalog :

GRL3001

quantity :

20 ug

price :

55 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant E.coli GroEL protein

Domain :

1-548aa

Catalog number (SKU) :

GRL3001

NCBI :

NP_418567.1

Description :

GroEL protein is the major heat shock protein of E. coli and belongs to the chaperonin (HSP60) family. GroEL protein prevents misfolding of proteins and promotes the refolding and proper assembly of unfolded polypeptiedes generated under stress condition. GroEL gene was amplified by PCR from E. coli and cloned into an expression vector. This protein was overexpressed in E. coli and was purified by using conventional chromatography techniques.

Alternative Names :

groL, groEL, mopA, Protein Cpn60, 60 kDa chaperonin, groEL protein, chaperonin groel

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 25mM Tris-HCl buffer (pH 7.5) containing 100mM NaCl, 5mM DTT, 10%glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Hemmingsen,S.M., et al (1988) Nature . 333(6171) 330-334;; Braig,K., et al(1994) Nature 371(6498) 578-586;; Chen,L and Singler,P.B. (1999) Cell 99(7) 757-768;;

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

E.coli

Gene Category :

Heat Shock Proteins

Molecular Weight :

57.3 kDa (548aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MAAKDVKFGN DARVKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPTIT KDGVSVAREI ELEDKFENMG AQMVKEVASK ANDAAGDGTT TATVLAQAII TEGLKAVAAG MNPMDLKRGI DKAVTAAVEE LKALSVPCSD SKAIAQVGTI SANSDETVGK LIAEAMDKVG KEGVITVEDG TGLQDELDVV EGMQFDRGYL SPYFINKPET GAVELESPFI LLADKKISNI REMLPVLEAV AKAGKPLLII AEDVEGEALA TLVVNTMRGI VKVAAVKAPG FGDRRKAMLQ DIATLTGGTV ISEEIGMELE KATLEDLGQA KRVVINKDTT TIIDGVGEEA AIQGRVAQIR QQIEEATSDY DREKLQERVA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALI RVASKLADLR GQNEDQNVGI KVALRAMEAP LRQIVLNCGE EPSVVANTVK GGDGNYGYNA ATEEYGNMID MGILDPTKVT RSALQYAASV AGLMITTECM VTDLPKNDAA DLGAAGGMGG MGGMGGMM

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P0A6F5

Internal Search Terms :

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more info or order :

NKMAX product webpage