protein

Recombinant human Growth Hormone 1/GH1, 20kDa variant protein

GRH3002

20 ug

55 USD

Recombinant human Growth Hormone 1/GH1, 20kDa variant protein

27-202aa

GRH3002

NP_072053.1

The 20KDa variant form of human growth hormone (20KDa hGH) presents in extracts from pituitary glands and it differs from the major form of hGH (22K, 191 amino acid) by the deletion of amino acid residues 32-46. The physiological role of 20K hGH remains to be determined partly but it regards the main function as stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues. Recombinant 20KDa human growth hormone was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Somatotropin isoform 2, 20kDa Growth Hormone, GH, GH1, GHN, GH-N, Growth hormone, Growth hormone 1, hGH-N, Pituitary growth hormone, Somatotropin

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Frigeri, et al (1979) Biochem Biophys Res Commun. 14:778-82;; Adelman, et al (1983) DNA. 2; 183-93;; Tsushima, et al (2000) Endocr J. 47; S17-21;; Horan, M.,et al(2003) Hum. Mutat. 21(4), 408-423;;

> 95% by SDS-PAGE

Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 range ≤1ng/ml.

SDS-PAGE, Bioactivity

Human

Hormones & Cytokines

20 kDa (177aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Human 20kDa Growth Hormone stimulates cell proliferation of the Nb2-11 Rat lymphoma cells. The ED50 range ≤1 ng/ml.

MFPTIPLSRL FDNAMLRAHR LHQLAFDTYQ EFNPQTSLCF SESIPTPSNR EETQQKSNLE LLRISLLLIQ SWLEPVQFLR SVFANSLVYG ASDSNVYDLL KDLEEGIQTL MGRLEDGSPR TGQIFKQTYS KFDTNSHNDD ALLKNYGLLY CFRKDMDKVE TFLRIVQCRS VEGSCGF

Non-Tagged

E.coli

Recombinant Protein

P01241

GH, GH 1, GH1, GHN, GH-N, Growth Hormone, Growth hormone 1, hGH-N, Pituitary growth hormone, Somatotropin, GRH3002-10ug, GRH3002-20ug, GRH3002-50ug, GRH3002-100ug, GRH3002-250ug, GRH3002-500ug, GRH3002-1mg, GRH3002-010, GRH3002-020, GRH3002-050, GRH3002-100, GRH3002-250, GRH3002-500, GRH3002-01M

Zhang H, et al. Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol-biotin chemiluminescent polymer. (Talanta. 2009){https://pubmed.ncbi.nlm.nih.gov/19576433/};;Jung J, et al. Production and engineering of nanobody-based quenchbody sensors for detecting recombinant human growth hormone and its isoforms. (Drug Test Anal. 2023){https://pubmed.ncbi.nlm.nih.gov/37667448/}