Recombinant E.coli G6PD protein | NKMAX GPD0905 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant E.coli G6PD protein

catalog :

GPD0905

quantity :

20 ug

price :

55 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant E.coli G6PD protein

Domain :

1-491aa

Catalog number (SKU) :

GPD0905

NCBI :

NP_416366.1

Description :

Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant G6PD protein was expressed in E. coli and purified by conventional chromatography techniques.

Alternative Names :

Glucose-6-phosphate 1-dehydrogenase, NADP(+)-dependent glucose-6-phosphate dehydrogenase, zwf, b1852, JW1841

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 0.1mM PMSF, 2mM EDTA, 0.5mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Huang Y., et al. (2008) Mol Genet Metab. 93(1):44-53.;; Zimny A., et al. (2003) Pol Arch Med Wewn. 110(5):1327-33;;

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 50unit/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

E.coli

Gene Category :

Enzymes & Proteases

Molecular Weight :

55.7 kDa (491aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 50 units/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.

Amino Acid Sequence :

MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P0AC53

Internal Search Terms :

G6PD, Glucose-6-phosphate 1-dehydrogenase, Glucose-6-phosphate 1-dehydrogenase, zwf, Glucose-6-phosphate 1-dehydrogenase G6PD, G6PD1, G6pdx, Glucose 6 phosphate 1 dehydrogenase, MET19, POS10, Zwf1p.,GPD0905-10ug,GPD0905-20ug,GPD0905-50ug,GPD0905-100ug,GPD0905-250ug,GPD0905-500ug,GPD0905-1mg,GPD0905-10,GPD0905-20,GPD0905-50,GPD0905-100,GPD0905-250,GPD0905-500,GPD0905-1

more info or order :

NKMAX product webpage