Recombinant saccharomyces cerevisiae GLC8 protein | NKMAX GLC0901 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant saccharomyces cerevisiae GLC8 protein

catalog :

GLC0901

quantity :

20 ug

price :

55 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant saccharomyces cerevisiae GLC8 protein

Domain :

1-229aa

Catalog number (SKU) :

GLC0901

NCBI :

NP_014042

Description :

GLC8 is a regulatory subunit of protein phosphatase 1 (Glc7p). This protein involved in glycogen metabolism and chromosome segregation, proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2. under normal conditions, Glc8p activates Glc7p, but when Glc8p is over produced, it may also inhibit Glc7p function. Glc8p is activated upon phosphoylation by Pho85p complexed with four cyclins (Pcl6p, Pcl7p, Pcl8p, or Pcl10p). Recombinant yeast GLC8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Glc8p, GLC8,

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Tung HY., et al. (1995) Mol Cell Biol. 15(11):6064-74;; Tan YS., et al. (2003) J Biol Chem. 278(1):147-53;;

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Saccharomyces cerevisiae

Gene Category :

Signal Transduction

Molecular Weight :

30.7 kDa (265aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Amino Acid Sequence :

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMGGI LKNPLALSPE QLAQQDPETL EEFRRQVYEN TQKNAKLTSH KRNIPGLDNT KEEGEIIGTS STFLPKDTLS LKHEQDMLAK MTPEERVQWN QRNLAENEIT KKQFQDIHID EPKTPYQGAV DPHGEYYRVD DDEDEDNSDK KPCQVANDDI DDLSLGEPEF EIKENKQPDF ETNDENDEDS PEARHKKFEE MRKKHYDVRA IFNKKSREAL KDEDEDEDDS TTKEP

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P41818

Internal Search Terms :

GLC8, Glc8p, GLC8,GLC0901-10ug,GLC0901-20ug,GLC0901-50ug,GLC0901-100ug,GLC0901-250ug,GLC0901-500ug,GLC0901-1mg,GLC0901-10,GLC0901-20,GLC0901-50,GLC0901-100,GLC0901-250,GLC0901-500,GLC0901-1

more info or order :

NKMAX product webpage