Recombinant human Elongin B protein | NKMAX ELB0901 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Elongin B protein

catalog :

ELB0901

quantity :

20 ug

price :

55 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Elongin B protein

Domain :

1-118aa

Catalog number (SKU) :

ELB0901

NCBI :

NP_009039

Description :

Elongin B, also known as TCEB2, is a subunit of the transcription factor B (SIII) complex. The SIII complex is a heterotrimer consisting of a transcriptionally active subunit (A) and two regulatory subunits (B and C). It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. The von Hippel-Lindau tumor suppressor protein (pVHL) binds to elongins B and C, and thereby inhibits transcription elongation. Recombinant TCEB2 was expressed as insoluble protein aggregate in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Alternative Names :

Elongin B, Transcription elongation factor B polypeptide 2, TCEB2, RNA polymerase II transcription factor SIII subunit B, SIII p18, EloB, Elongin 18 kDa subunit, EloB, TCEB 2, Transcription elongation factor B (SIII) polypeptide 2.

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1mM PMSF, 20% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Piessevaux J., et al. (2008) J Biol Chem. 283(31):21334-46.;; Duan DR., et al. (1995) Science. 269(5229):1402-6;;

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Cell Cycle

Molecular Weight :

13.1 kDa (118aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MDVFLMIRRH KTTIFTDAKE SSTVFELKRI VEGILKRPPD EQRLYKDDQL LDDGKTLGEC GFTSQTARPQ APATVGLAFR ADDTFEALCI EPFSSPPELP DVMKPQDSGS SANEQAVQ

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q15370

Internal Search Terms :

Elongin B, Elongin B, Transcription elongation factor B polypeptide 2, TCEB2, RNA polymerase II transcription factor SIII subunit B, SIII p18, EloB, Elongin 18 kDa subunit, EloB, TCEB 2, Transcription elongation factor B (SIII) polypeptide 2.,ELB0901-10ug,ELB0901-20ug,ELB0901-50ug,ELB0901-100ug,ELB0901-250ug,ELB0901-500ug,ELB0901-1mg,ELB0901-10,ELB0901-20,ELB0901-50,ELB0901-100,ELB0901-250,ELB0901-500,ELB0901-1

more info or order :

NKMAX product webpage