Recombinant human EGF protein | NKMAX EGF0501 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human EGF protein

catalog :

EGF0501

quantity :

20 ug

price :

25 USD

more info or order :

NKMAX product webpage

citations: 1

Reference
Tazawa H, Kawaguchi T, Kobayashi T, Kuramitsu Y, Wada S, Satomi Y, et al. Chronic inflammation-derived nitric oxide causes conversion of human colonic adenoma cells into adenocarcinoma cells. Exp Cell Res. 2013;319:2835-44 pubmed publisher

product information

Product Type :

Proteins

Item Name :

Recombinant human EGF protein

Domain :

971-1023aa

Catalog number (SKU) :

EGF0501

NCBI :

NP_001954.2

Description :

Recombinant human epidermal growth factor (EGF) is a 6. 3 kDa globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Alternative Names :

Epidermal growth factor, Pro-epidermal growth factor, urogastrone, EGF

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Riese., et al (1998 ) Bioessays. 20: 41-48.;; Cohen S., (1983) Cancer. 15: 1787-1791. ;; Carpenter G., et al (1979) Annu. Rev. Biochem., 48: 193-216.;;

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is less or equal to 0.1ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Hormones & Cytokines

Molecular Weight :

6.3 kDa (54aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human EGF stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cells. The ED50 range is less or equal to 0.1 ng/ml.

Amino Acid Sequence :

MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P01133

Internal Search Terms :

EGF,EGF0501-10ug,EGF0501-20ug,EGF0501-50ug,EGF0501-100ug,EGF0501-250ug,EGF0501-500ug,EGF0501-1mg,EGF0501-10,EGF0501-20,EGF0501-50,EGF0501-100,EGF0501-250,EGF0501-500,EGF0501-1, epidermal growth factor (beta-urogastrone)

References with this Product :

Tazawa H, et al. Chronic inflammation-derived nitric oxide causes conversion of human colonic adenoma cells into adenocarcinoma cells. (Exp Cell Res. 2013){https://pubmed.ncbi.nlm.nih.gov/23948305/};;Park SY, et al. DNAJB1 negatively regulates MIG6 to promote epidermal growth factor receptor signaling. (Biochim Biophys Acta. 2015){https://pubmed.ncbi.nlm.nih.gov/26239118/};;Okuda K, et al. Pivotal role for S-nitrosylation of DNA methyltransferase 3B in epigenetic regulation of tumorigenesis. (Nat Commun. 2023){https://pubmed.ncbi.nlm.nih.gov/36739439/}

more info or order :

NKMAX product webpage