protein

Recombinant E.coli Dnak(508-638aa) protein

DNK3004

20 ug

55 USD

Recombinant E.coli Dnak(508-638aa) protein

508-638aa

DNK3004

NP_414555

DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. Dnak (residues 508-638) of the substrate binding domain is alpha-helical and appears to act as a lid covering the substrate binding cleft. DnaK (amino acid 508-638) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus

Dnak (508-638), A lid covering the substrate, Heat shock protein 70, Heat shock 70 kDa protein, HSP70, Chaperone protein dnaK, Chaperone Hsp 70, Co chaperone with DnaJ, dnaK, Heat shock 70 kDa protein,

1mg/ml (determined by BCA assay)

Liquid in. 25mM Tris-HCl buffer (pH 7.5) containing 100mM NaCl, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bardwell & Craig (1984) Proc. Natl. Acad. Sci. 81, 848-852;; Zhu et al., (1996) Science 272, 1606-1614.;; Naoki tanaka., et al (2002) PNAS 26(99)15398-15403;;

> 95% by SDS-PAGE

SDS-PAGE

E.coli

Heat Shock Proteins

14.6 kDa (132aa)

MNEDEIQKMV RDAEANAEAD RKFEELVQTR NQGDHLLHST RKQVEEAGDK LPADDKTAIE SALTALETAL KGEDKAAIEA KMQELAQVSQ KLMEIAQQQH AQQQTAGADA SANNAKDDDV VDAEFEEVKD KK

Non-Tagged

E.coli

Recombinant Protein

P0A6Y8

Dnak, Dnak (508-638), A lid covering the substrate, Heat shock protein 70, Heat shock 70 kDa protein, HSP70, Chaperone protein dnaK, Chaperone Hsp 70, Co chaperone with DnaJ, dnaK, Heat shock 70 kDa protein,DNK3004-10ug,DNK3004-20ug,DNK3004-50ug,DNK3004-100ug,DNK3004-250ug,DNK3004-500ug,DNK3004-1mg,DNK3004-10,DNK3004-20,DNK3004-50,DNK3004-100,DNK3004-250,DNK3004-500,DNK3004-1