Recombinant E.coli Dnak(385-546aa) protein | NKMAX DNK2001 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant E.coli Dnak(385-546aa) protein

catalog :

DNK2001

quantity :

20 ug

price :

55 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant E.coli Dnak(385-546aa) protein

Domain :

385-546aa

Catalog number (SKU) :

DNK2001

NCBI :

NP_414555

Description :

DNAK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus

Alternative Names :

Chaperone protein dnaK, HSP70, groP, grpF, seg, Heat shock protein 70, Chaperone Hsp70, Co chaperone with DnaJ, dnaK, Heat shock 70 kDa protein,

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 25mM Tris-HCl buffer (pH 7.5) containing 2mM beta-mercaptoethanol, 1mM EDTA

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Bardwell & Craig (1984) Proc. Natl. Acad. Sci. 81, 848-852;; Zhu et al., (1996) Science 272, 1606-1614.;;

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

E.coli

Gene Category :

Heat Shock Proteins

Molecular Weight :

17.7 kDa (163aa)

Amino Acid Sequence :

MDVKDVLLLD VTPLSLGIET MGGVMTTLIA KNTTIPTKHS QVFSTAEDNQ SAVTIHVLQG ERKRAADNKS LGQFNLDGIN PAPRGMPQIE VTFDIDADGI LHVSAKDKNS GKEQKITIKA SSGLNEDEIQ KMVRDAEANA EADRKFEELV QTRNQGDHLL HST

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P0A6Y8

Internal Search Terms :

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more info or order :

NKMAX product webpage