Recombinant E.coli DnaJ protein | NKMAX DNJ3001 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant E.coli DnaJ protein

catalog :

DNJ3001

quantity :

20 ug

price :

55 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant E.coli DnaJ protein

Domain :

1-376aa

Catalog number (SKU) :

DNJ3001

NCBI :

NP_414556

Description :

DnaJ, Heat shock protein, functions in association with DnaK (Hsp70) molecular chaperone to facilitate protein folding. p70 chaperone. DnaJ plays a key role in the chaperone reaction by stimulating the ATPase activity and activating the substrate binding of Hsp70. DnaJ consists of four domains that are N-terminal 76 amino acid J-domain, G/F domain, zinc-binding cystein rich CR-domain, C-terminal CTD-domain and they are conserved to various degrees among the homologues. DnaJ (amino acids 1-376) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques.

Alternative Names :

DNAJA1, DNAJ2, HDJ2, HSJ2, HSPF4, DnaJ (1-376aa), Heat shock 40 kDa protein 4, DnaJ protein homolog 2, HDJ-2, HSJ-2, HSDJ, DnaJ homolog subfamily A member 1,

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 25mM Tris-HCl buffer (pH 8.8) containing 5mM DTT, 100mM NaCl, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Bardwell,J.C., et al (1986) J.Biol.Chem.261(4);;1782-1785;; Ohki M.,et al (1986) J.Biol.Chem.261(4);;1778-1781;; Zylicz M., et.al.(1989) EMBO J 8(5)1601-8 ;; Landry SJ., et al. (2003) Biochemistry 42(17)4926-36;;

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

E.coli

Gene Category :

Heat Shock Proteins

Molecular Weight :

41.1 kDa (376aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MAKQDYYEIL GVSKTAEEHE IRKAYKRLAM KYHPDRNQGD KEAEAKFKEI KEAYEVLTDS QKRAAYDQYG HAAFEQGGMG GGGFGGGADF SDIFGDVFGD IFGGGRGRQR AARGADLRYN MELTLEEAVR GVTKEIRIPT LEECDVCHGS GAKPGTQPQT CPTCHGSGQV QMRQGFFAVQ QTCPHCQGRG TLIKDPCNKC HGHGRVERSK TLSVKIPAGV DTGDRIRLAG EGEAGEHGAP AGDLYVQVQV KQHPIFEREG NNLYCEVPIN FAMAALGGEI EVPTLDGRVK LKVPGETQTG KLFRMRGKGV KSVRGGAQGD LLCRVVVETP VGLNERQKQL LQELQESFGG PTGEHNSPRS KSFFDGVKKF FDDLTR

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P08622

Internal Search Terms :

DnaJ, DNAJA1, DNAJ2, HDJ2, HSJ2, HSPF4, DnaJ (1-376aa), Heat shock 40 kDa protein 4, DnaJ protein homolog 2, HDJ-2, HSJ-2, HSDJ, DnaJ homolog subfamily A member 1,DNJ3001-10ug,DNJ3001-20ug,DNJ3001-50ug,DNJ3001-100ug,DNJ3001-250ug,DNJ3001-500ug,DNJ3001-1mg,DNJ3001-10,DNJ3001-20,DNJ3001-50,DNJ3001-100,DNJ3001-250,DNJ3001-500,DNJ3001-1

more info or order :

NKMAX product webpage