protein

Recombinant human Cyclophilin B/PPIB protein

CYP0701

20 ug

55 USD

Recombinant human Cyclophilin B/PPIB protein

26-216aa

CYP0701

NP_000933

Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Recombinant human cyclophilin B was expressed in E. coli and purified by using conventional chromatography techniques

Peptidyl-prolyl cis-trans isomerase B, Peptidylprolyl isomerase B, PPIase B, Cphn-2, Cyclophilin B, CYP-20b, CYPB, CYP-S1, OI9, Rotamase B, S-cyclophilin, SCYLP

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20mM NaCl, 0.5mM DTT, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Watashi K, et al.(2005) Mol Cell. 19(1):111-22. ;; Obata Y, et al. (2005) J Biol Chem. 280(18):18355-60;; Denys A , et al. (1997) Immunology. 91(4):609-17.;;

> 95% by SDS-PAGE

Specific activity is > 1,500nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

SDS-PAGE, Enzyme Activity

Human

Cell Cycle

21.2 kDa (192aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 1,500 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

MLLPGPSAAD EKKKGPKVTV KVYFDLRIGD EDVGRVIFGL FGKTVPKTVD NFVALATGEK GFGYKNSKFH RVIKDFMIQG GDFTRGDGTG GKSIYGERFP DENFKLKHYG PGWVSMANAG KDTNGSQFFI TTVKTAWLDG KHVVFGKVLE GMEVVRKVES TKTDSRDKPL KDVIIADCGK IEVEKPFAIA KE

Non-Tagged

E.coli

Recombinant Protein

P23284

26- 216aa, 26-216aa, AA408962, AA553318, AI844835, Cphn 2, Cphn2, Cphn-2, Cyclophilin B, Cyclophilin-B, CyclophilinB, CyP 20b, CyP-20b, CyP20b, CYP S1, CYP-S1, CYPS1, CYPB, CYP-S1: Cyclophilin-B, MGC14109, MGC2224, Peptidyl prolyl cis trans isomerase B, Peptidyl prolyl cis trans isomerase B precursor, Peptidyl-prolyl cis-trans isomerase B, Peptidylprolyl isomerase B, PPIase, PPIB, Rotamase, S cyclophilin, SCYLP, CYP0701-10ug, CYP0701-20ug, CYP0701-50ug, CYP0701-100ug, CYP0701-250ug, CYP0701-500ug, CYP0701-1mg, CYP0701-010, CYP0701-020, CYP0701-050, CYP0701-100, CYP0701-250, CYP0701-500, CYP0701-01M

Kim K, et al. Release of overexpressed CypB activates ERK signaling through CD147 binding for hepatoma cell resistance to oxidative stress. (Apoptosis. 2012){https://pubmed.ncbi.nlm.nih.gov/22555451/}