protein

Recombinant human G-CSF protein

CSF4001

10 ug

80 USD

Recombinant human G-CSF protein

31-204aa

CSF4001

NP_757373.1

Human granulocyte colony-stimulating factor (hG-CSF) is one of the hematopoietic growth factors which plays an important role in stimulating proliferation, differentiation, and functional activation of blood cells. In addition to its effect on hematopoiesis, G-CSF also enhances neutrophil functions against bacteria, fungi, and tumor cells mediated by antibody-dependent cell-mediated cytotoxicity. Recombinant hG-CSF was expressed in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Granulocyte-colony stimulating factor, CSF3, GCSF, G-CSF, G-CSF, Pluripoietin, Filgrastim, Lenograstim, Granulocyte colony-stimulating factor, Granulocyte-colony stimulating factor Colony stimulating factor 3 (granulocyte), CSF 3, CSF beta, Csfg, GCSA, Granulocyte colony stimulating factor, Lenograstim, Macrophage granulocyte inducer 2, MGC45931, MGI 2,

0.5mg/ml (determined by Bradford assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Nomura H, et al.(1986) EMBO J , 5(5): 871-6;; Nagata, S., et al.(1986) Nature, 319 : 415-418;; Demetri, G. D., et al.(1991) Blood, 78: 2791-2808 ;; Hill, C. P., et al (1993) Proc. Natl. Acad. Sci. uSA, 90:5167-5171;;

> 95% by SDS-PAGE

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤0.3ng/ml.

SDS-PAGE, Bioactivity

Human

Hormones & Cytokines

18.8 kDa (175aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Human G-CSF stimulates cell proliferation of the M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤0.3 ng/ml.

MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP

Non-Tagged

E.coli

Recombinant Protein

P09919

G-CSF,CSF4001-10ug,CSF4001-20ug,CSF4001-50ug,CSF4001-100ug,CSF4001-250ug,CSF4001-500ug,CSF4001-1mg,CSF4001-10,CSF4001-20,CSF4001-50,CSF4001-100,CSF4001-250,CSF4001-500,CSF4001-1

Lee EN, et al. Stabilizing peptide fusion for solving the stability and solubility problems of therapeutic proteins. Pharm Res. 2005 Oct;22(10):1735-46.{https://pubmed.ncbi.nlm.nih.gov/16180132/}