protein

Recombinant human Crystallin alpha A/CRYAA protein

CRA3001

1 mg

480 USD

Recombinant human Crystallin alpha A/CRYAA protein

1-173aa

CRA3001

NP_000385.1

Alpha crystallins are composed of two gene products ; alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20). They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function of alpha-crystallins are an autokinase activity and participation in the intracellular architecture. The expression of alpha-A is preferentially restricted to the lens cell.

Acry-1, Alpha crystallin A chain, CRYA1, CRYAA, Crystallin alpha 1, Crystallin alpha A, Heat shock protein beta 4, HSPB 4, HspB4, Zonular Central Nuclear Cataract

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 50mM NaCl, 1mM EDTA

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Hasan, A., et al.(2002) Biochemistry 41(52) 15876-15882;; Bera,S., et al(2002) Biochemistry 41(41) 12421-12426 ;; Reddy, G.B., et al(2002) FEBS lett. 522(1-3) 59-64 ;; Andley, u.P., et al(2002) J.Biol.Chem.277(12) 10178-10186;;

> 90% by SDS-PAGE

SDS-PAGE

Human

Heat Shock Proteins

19.9 kDa (173aa)

MDVTIQHPWF KRTLGPFYPS RLFDQFFGEG LFEYDLLPFL SSTISPYYRQ SLFRTVLDSG ISEVRSDRDK FVIFLDVKHF SPEDLTVKVQ DDFVEIHGKH NERQDDHGYI SREFHRRYRL PSNVDQSALS CSLSADGMLT FCGPKIQTGL DATHAERAIP VSREEKPTSA PSS

Non-Tagged

E.coli

Recombinant Protein

P02489

a A Crystallin, Acry-1, Alpha crystallin A chain, Alpha-crystallin A chain, CRYA 1, CRYA1, CRYAA, Crystallin Alpha 1, Crystallin alpha A, Heat shock protein beta 4, Heat shock protein beta-4, HSPB 4, HspB4, Zonular Central Nuclear Cataract, CRA3001-10ug, CRA3001-20ug, CRA3001-50ug, CRA3001-100ug, CRA3001-250ug, CRA3001-500ug, CRA3001-1mg, CRA3001-010, CRA3001-020, CRA3001-050, CRA3001-100, CRA3001-250, CRA3001-500, CRA3001-01M

Fan Q, et al. Identification of proteins that interact with alpha A-crystallin using a human proteome microarray. (Mol Vis. 2014){https://pubmed.ncbi.nlm.nih.gov/24453475/}