protein

Recombinant human COMT protein

COM0905

20 ug

160 USD

Recombinant human COMT protein

51-271aa

COM0905

NP_000745.1

Catechol-O-methyltrasferase (COMT) is an enzyme that catalyses the transfer of the methyl group of S-adenosylmethionine (SAM) into catechol substrates such as levodopa, dopamine, epinephrine, and norepinephirine in the presence of Mg2+. COMT appears to be located in the postsynaptic neuron and plays roles in the metabolism of catechol estrogens and the inactivation of catecholamine neurotransmitters though enzymatic degradation. COMT inhibitors prevent levodopa degradation, increase its availability and are used in the treatment of patients with Parkinson's disease. Recombinant human COMT protein was expressed in E. coli and purified by using conventional chromatography techniques.

Catechol-O-methyltransferase, COMT

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM MgCl2, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Cotton NJ., et al. (2004) J Biol Chem. 279(22):23710-8. ;; Dawling S., et al. (2001) Cancer Res. 61(18):6716-22.;;

> 95% by SDS-PAGE

SDS-PAGE

Human

Enzymes & Proteases

24.4 kDa (221aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

MGDTKEQRIL NHVLQHAEPG NAQSVLEAID TYCEQKEWAM NVGDKKGKIV DAVIQEHQPS VLLELGAYCG YSAVRMARLL SPGARLITIE INPDCAAITQ RMVDFAGVKD KVTLVVGASQ DIIPQLKKKY DVDTLDMVFL DHWKDRYLPD TLLLEECGLL RKGTVLLADN VICPGAPDFL AHVRGSSCFE CTHYQSFLEY REVVDGLEKA IYKGPGSEAG P

Non-Tagged

E.coli

Recombinant Protein

P21964

COMT, Catechol-O-methyltransferase, Catechol-O-methyltransferase, Catechol-O-methyltransferase, COMT,COM0905-10ug,COM0905-20ug,COM0905-50ug,COM0905-100ug,COM0905-250ug,COM0905-500ug,COM0905-1mg,COM0905-10,COM0905-20,COM0905-50,COM0905-100,COM0905-250,COM0905-500,COM0905-1

Ishida Y, et al. Yokukansan, a Traditional Japanese Medicine, Enhances the L-DOPA-Induced Rotational Response in 6-Hydroxydopamine-Lesioned Rats: Possible Inhibition of COMT. (Biol Pharm Bull. 2016){https://pubmed.ncbi.nlm.nih.gov/29707715/};;Ikeda M, et al. Inhibitory Effect of Bovine Lactoferrin on Catechol-O-Methyltransferase. (Molecules. 2017){https://pubmed.ncbi.nlm.nih.gov/28825621/};;Ikeda M, et al. Effects of bovine lactoferrin on l-DOPA absorption and metabolism in mice. (Food Funct. 2018){https://pubmed.ncbi.nlm.nih.gov/26725433/};;Nagai M, et al. Comparative examination of levodopa pharmacokinetics during simultaneous administration with lactoferrin in healthy subjects and the relationship between lipids and COMT inhibitory activity in vitro. (Nutr Neurosci. 2020){https://pubmed.ncbi.nlm.nih.gov/32441567/}