protein

Recombinant human CHIP/STUB1 protein

CHP0905

20 ug

55 USD

Recombinant human CHIP/STUB1 protein

1-303aa

CHP0905

NP_005852

CHIP, also known as STuB1, is a cytoplasmic protein whose amino acid sequence is highly conserved across species. CHIP interacts with the molecular chaperones Hsc70-Hsp70 and Hsp90 through its TPR domain, whereas its u-box domain contains its E3 ubiquitin ligase activity. Its interaction with these molecular chaperones results in client substrate ubiquitylation and degradation by the proteasome. Thus, CHIP acts to tilt the folding-refolding machinery toward the degradative pathway, and it serves as a link between the two. Recombinant human CHIP protein was expressed in E. coli and purified by using conventional chromatography techniques.

Carboxy terminus of HSP70 interacting protein, STuB1, HSPABP2, NY-CO-7, SDCCAG7, uBOX1, Carboxy terminus of HSP70 interacting protein Antigen NY CO 7, CLL associated antigen KW 8, Carboxy terminus of Hsp70p interacting protein, CHIP, E3 ubiquitin protein ligase CHIP, STuB 1, uBOX 1, Heat shock protein A binding protein 2 (c terminal), NY CO 7, Serologically defined colon cancer antigen 7, STIP1 homology and u Box containing protein 1.

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 5mM DTT, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Kajiro M., et al. (2009) Nat Cell Biol. 11(3):312-9.;; Ko HS., et al. (2009) Proc Natl Acad Sci. 106(8):2897-902.;;

> 90% by SDS-PAGE

SDS-PAGE

Human

Heat Shock Proteins

34.8 kDa (303aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

MKGKEEKEGG ARLGAGGGSP EKSPSAQELK EQGNRLFVGR KYPEAAACYG RAITRNPLVA VYYTNRALCY LKMQQHEQAL ADCRRALELD GQSVKAHFFL GQCQLEMESY DEAIANLQRA YSLAKEQRLN FGDDIPSALR IAKKKRWNSI EERRIHQESE LHSYLSRLIA AERERELEEC QRNHEGDEDD SHVRAQQACI EAKHDKYMAD MDELFSQVDE KRKKRDIPDY LCGKISFELM REPCITPSGI TYDRKDIEEH LQRVGHFDPV TRSPLTQEQL IPNLAMKEVI DAFISENGWV EDY

Non-Tagged

E.coli

Recombinant Protein

Q9UNE7

CHIP, STUB1, Carboxy terminus of HSP70 interacting protein, STuB1, HSPABP2, NY-CO-7, SDCCAG7, uBOX1, Carboxy terminus of HSP70 interacting protein Antigen NY CO 7, CLL associated antigen KW 8, Carboxy terminus of Hsp70p interacting protein, CHIP, E3 ubiquitin protein ligase CHIP, STuB 1, uBOX 1, Heat shock protein A binding protein 2 (c terminal), NY CO 7, Serologically defined colon cancer antigen 7, STIP1 homology and u Box containing protein 1.,CHP0905-10ug,CHP0905-20ug,CHP0905-50ug,CHP0905-100ug,CHP0905-250ug,CHP0905-500ug,CHP0905-1mg,CHP0905-10,CHP0905-20,CHP0905-50,CHP0905-100,CHP0905-250,CHP0905-500,CHP0905-1