Human Wnt-3a antibody | NKMAX AWN0924 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Wnt-3a antibody

catalog :

AWN0924

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3A6

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Wnt-3a antibody

Catalog number (SKU) :

AWN0924

NCBI :

NP_149122

Description :

Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctioanl factors that play a role in both tumorigenesis and patterning events required for normal differentiation and development. Wnt3a is a ligand for members of the frizzled family of seven transmembrane receptors.

Alternative Names :

Wnt3a, Wingless-type MMTV integration site family, member 3A

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Pan W., et al. (2008) Science. 321(5894):1350-1353.;; Katoh M., et al. (2002) Int J Oncol. 20(2):373-377.;; Smolich B D., et al. (1993) Mol Biol Cell. 4(12):1267-1275.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human Wnt3a (19-352aa) purified from E. coli

Applications :

ELISA, WB, IHC

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Wnt3a antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: 293T cell lysate;; Lane 2.: Wnt3a Transfected 293T cell lysate

Reacts With :

Human

Clone Number :

3A6

Ab Usage :

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

IHC Comment And Pic :

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human Wnt3a (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Antigen Species :

Human

Gene Category :

Development

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P56704

Internal Search Terms :

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more info or order :

NKMAX product webpage