Human Cellubrevin/VAMP-3 antibody | NKMAX AVA0922 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Cellubrevin/VAMP-3 antibody

catalog :

AVA0922

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4G9

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Cellubrevin/VAMP-3 antibody

Catalog number (SKU) :

AVA0922

NCBI :

NP_004772

Description :

Cellubrevin, also known as VAMP3, is a member of the Vamp/synaptobrevin family. Vamps, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Cellubrevin is a vesicular soluble N-ethylmaleimide sensitive factor attachment protein receptor (v-SNARE) homologous to the neuronal synaptobrevins 1/2 and is also a substrate of tetanus neurotoxin.

Alternative Names :

Vesicle-associated membrane protein 3, VAMP 3, CEB, Synaptobrevin-3, Cellubrevin, Synaptobrevin 3, VAMP3, Vesicle associated membrane protein 3

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Proux-G V., et al. (2005) Proc Natl Acad Sci USA. 102(18):6362-6367.;; Randhawa Vk., et al. (2000) Mol Cell Biol. 11(7):2403-2417.;; Olson AL., et al. (1997) Mol Cell Biol. 17(5):2425-2435.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human cellubrevin (1-77aa) purified from E. coli

Applications :

ELISA, WB, FACS

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human cellubrevin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: Cellubrevin transfected 293T cell lysate The mouse brain tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human cellubrevin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {AVA0922-WB2.jpg}

Reacts With :

Human

Clone Number :

AT4G9

Ab Usage :

The antibody has been tested by ELISA, Western blot and Flow cytometry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of Cellubrevin in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Neuroscience

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q15836

Internal Search Terms :

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more info or order :

NKMAX product webpage