Human VAMP-2 antibody | NKMAX AVA0710 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human VAMP-2 antibody

catalog :

AVA0710

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3E5

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human VAMP-2 antibody

Catalog number (SKU) :

AVA0710

NCBI :

NP_055047

Description :

Synaptobrevin 2 (Vesicle-associated membrane, VAMP2), which is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal tail. Synaptobrevin 2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25. This antibody recognizes specifically synaptobrevin 2, but it also shows a low affinity to synaptobrevin 1.

Alternative Names :

vesicle-associated membrane protein 2, VAMP2, SYB2, Synaptobrevin 2, VAMP-2, Synaptobrevin-2, Vesicle-associated membrane protein 2, vesicle-associated membrane protein 2 FLJ11460, RATVAMPB, RATVAMPIR, SYB, VAMP 2, Vesicle associated membrane protein 2, Vesicle-associated membrane protein 2 (synaptobrevin 2)

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Andres C et al.,(1995) Proc Natl Acad Sci USA 92:5987-5991.;; Jacobsson G et al.,(1998) European Journal of Neuroscience 16:301-316.;; Scales SJ. et al (2002) J Biol. Chem. 227(31):28271-28279.;; Pennuto M et al., (2003) Mol Biol Cell. 14:4909-19.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human Synaptobrevin 2 (1-89aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of VAMP-2 in U87MG cells. The cell was stained with AVA0710 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VAMP-2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human VAMP1 protein;;Lane 2.: Recombinant human VAMP2 protein;;Lane 3.: Recombinant human VAMP3 protein;;The tissue lysate (50ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VAMP-2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse brain Tissue lysate{AVA0710-WB2.jpg}The tissue lysate (20ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VAMP-2 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Rat brain Tissue lysate{AVA0710-WB3.jpg}

Reacts With :

Human, Mouse

Clone Number :

3E5

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P63027

Internal Search Terms :

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more info or order :

NKMAX product webpage